Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays

Joint Authors

Shipman, Richard D.
Doering, Sean D.
Hemsath, Jack R.
Lee, Eun Joo
Grant, Jennifer E.

Source

Biochemistry Research International

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-10-26

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Chemistry

Abstract EN

Phosvitin, the most highly phosphorylated metal-binding protein found in nature, binds more than 100 calcium ions, and has been identified as an agent that could be used to generate biomineralization scaffolds.

Because of published reports describing phosvitin’s affinity for calcium and potential antibiotic activity, this study was undertaken in order to evaluate phosvitin for both antibiotic activity against common microorganisms and the ability to protect hydroxyapatite surfaces from acid damage.

To more clearly define its antibiotic action, the effects of phosvitin on Micrococcus luteus, P.

mirabilis, B.

cereus, E.

coli, and S.

epidermidis were evaluated.

In both Kirby–Bauer tests and liquid culture growth inhibition assays, phosvitin inhibited M.

luteus, a microorganism that thrives in the human mouth, but not the other bacteria tested.

The MIC of phosvitin was determined to be 31.3 μg/mL when delivered in 1 mM CaCl2 but was 0.5 mg/mL in the absence of added calcium.

Expanding on the potential impacts of phosvitin on the mouth, its action was evaluated in a model of tooth decay represented by acid-damaged hydroxyapatite discs.

SEM, AFM, and FAAS analyses revealed that pretreatment of discs with phosvitin modulated the damage-induced morphology and topography changes associated with acid-damaged discs.

American Psychological Association (APA)

Shipman, Richard D.& Doering, Sean D.& Hemsath, Jack R.& Lee, Eun Joo& Grant, Jennifer E.. 2020. Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays. Biochemistry Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138473

Modern Language Association (MLA)

Shipman, Richard D.…[et al.]. Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays. Biochemistry Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1138473

American Medical Association (AMA)

Shipman, Richard D.& Doering, Sean D.& Hemsath, Jack R.& Lee, Eun Joo& Grant, Jennifer E.. Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays. Biochemistry Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1138473

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1138473