Identifying the Genes Responsible for Iron-Limited Condition in Riemerella anatipestifer CH-1 through RNA-Seq-Based Analysis
Joint Authors
Chen, Shun
Wang, Mingshu
Zhu, Dekang
Liu, Mafeng
Cheng, Anchun
Jia, Renyong
Yang, Qiao
Wu, Ying
Sun, Kunfeng
Huang, Mi
Biville, Francis
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
One of the important elements for most bacterial growth is iron, the bioavailability of which is limited in hosts.
Riemerella anatipestifer (R.
anatipestifer, RA), an important duck pathogen, requires iron to live.
However, the genes involved in iron metabolism and the mechanisms of iron transport are largely unknown.
Here, we investigated the transcriptomic effects of iron limitation condition on R.
anatipestifer CH-1 using the RNA-Seq and RNA-Seq-based analysis.
Data analysis revealed genes encoding functions related to iron homeostasis, including a number of putative TonB-dependent receptor systems, a HmuY-like protein-dependent hemin (an iron-containing porphyrin) uptake system, a Feo system, a gene cluster related to starch utilization, and genes encoding hypothetical proteins that were significantly upregulated in response to iron limitation.
Compared to the number of upregulated genes, more genes were significantly downregulated in response to iron limitation.
The downregulated genes mainly encoded a number of outer membrane receptors, DNA-binding proteins, phage-related proteins, and many hypothetical proteins.
This information suggested that RNA-Seq-based analysis in iron-limited medium is an effective and fast method for identifying genes involved in iron uptake in R.
anatipestifer CH-1.
American Psychological Association (APA)
Liu, Mafeng& Huang, Mi& Zhu, Dekang& Wang, Mingshu& Jia, Renyong& Chen, Shun…[et al.]. 2017. Identifying the Genes Responsible for Iron-Limited Condition in Riemerella anatipestifer CH-1 through RNA-Seq-Based Analysis. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139220
Modern Language Association (MLA)
Liu, Mafeng…[et al.]. Identifying the Genes Responsible for Iron-Limited Condition in Riemerella anatipestifer CH-1 through RNA-Seq-Based Analysis. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139220
American Medical Association (AMA)
Liu, Mafeng& Huang, Mi& Zhu, Dekang& Wang, Mingshu& Jia, Renyong& Chen, Shun…[et al.]. Identifying the Genes Responsible for Iron-Limited Condition in Riemerella anatipestifer CH-1 through RNA-Seq-Based Analysis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139220
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139220