Effectiveness of a Lytic Phage SRG1 against Vancomycin-Resistant Enterococcus faecalis in Compost and Soil
Joint Authors
Rahmat Ullah, Sidra
Andleeb, Saadia
Raza, Taskeen
Jamal, Muhsin
Mehmood, Khalid
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Nosocomial infections caused by vancomycin-resistant Enterococcus have become a major problem.
Bacteriophage therapy is proposed as a potential alternative therapy.
Bacteriophages are viruses that infect bacteria and are ubiquitous in nature.
Lytic bacteriophage was isolated from sewage water that infects VREF, the causative agent of endocarditis, bacteraemia, and urinary tract infections (UTIs).
The phage produced clear plaques with unique clear morphology and well-defined boundaries.
TEM results of phage revealed it to be 108±0.2 nm long and 90±0.5 nm wide.
The characterization of bacteriophage revealed that infection process of phage was calcium and magnesium dependent and phage titers were highest under optimum conditions for VREF, with an optimal temperature range of 37–50°C.
The maximum growth was observed at 37°C, hence having 100% viability.
The latent period for phage was small with a burst size of 512 viral particles per bacterial cell.
The phage was tested against various clinical strains and results proved it to be host specific.
It can be used as a potential therapeutic agent for VREF infections.
The phage efficiently eradicated VREF inoculated in cattle compost, poultry compost, and a soil sample which makes it a potential agent for clearing compost and soil sample.
American Psychological Association (APA)
Rahmat Ullah, Sidra& Andleeb, Saadia& Raza, Taskeen& Jamal, Muhsin& Mehmood, Khalid. 2017. Effectiveness of a Lytic Phage SRG1 against Vancomycin-Resistant Enterococcus faecalis in Compost and Soil. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139488
Modern Language Association (MLA)
Rahmat Ullah, Sidra…[et al.]. Effectiveness of a Lytic Phage SRG1 against Vancomycin-Resistant Enterococcus faecalis in Compost and Soil. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139488
American Medical Association (AMA)
Rahmat Ullah, Sidra& Andleeb, Saadia& Raza, Taskeen& Jamal, Muhsin& Mehmood, Khalid. Effectiveness of a Lytic Phage SRG1 against Vancomycin-Resistant Enterococcus faecalis in Compost and Soil. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139488