Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection

Joint Authors

Lü, Dingding
Hou, Chengxiang
Qin, Guangxing
Gao, Kun
Chen, Tian
Guo, Xijie

Source

BioMed Research International

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-01-17

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Medicine

Abstract EN

A full-length cDNA of lebocin 5 (BmLeb5) was first cloned from silkworm, Bombyx mori, by rapid amplification of cDNA ends.

The BmLeb5 gene is 808 bp in length and the open reading frame encodes a 179-amino acid hydroxyproline-rich peptide.

Bioinformatic analysis results showed that BmLeb5 owns an O-glycosylation site and four RXXR motifs as other lebocins.

Sequence similarity and phylogenic analysis results indicated that lebocins form a multiple gene family in silkworm as cecropins.

Quantitative real-time PCR analysis revealed that BmLeb5 was highest expressed in the fat body.

In the silkworm larvae infected by Beauveria bassiana, the expression level of BmLeb5 was upregulated in the fat body and hemolymph which are the most important immune tissues in silkworm.

The recombinant protein of BmLeb5 was for the first time successfully expressed with prokaryotic expression system and purified.

There are no reports so far that the expression of lebocins could be induced by entomopathogenic fungus.

Our study suggested that BmLeb5 might play an important role in the immune response of silkworm to defend B.

bassiana infection.

The results also provided helpful information for further studying the lebocin family functioned in antifungal immune response in the silkworm.

American Psychological Association (APA)

Lü, Dingding& Hou, Chengxiang& Qin, Guangxing& Gao, Kun& Chen, Tian& Guo, Xijie. 2017. Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139518

Modern Language Association (MLA)

Lü, Dingding…[et al.]. Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139518

American Medical Association (AMA)

Lü, Dingding& Hou, Chengxiang& Qin, Guangxing& Gao, Kun& Chen, Tian& Guo, Xijie. Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139518

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1139518