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DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes
Joint Authors
Lee, Guem-San
Doh, Eui-Jeong
Oh, Seung-Eun
Yun, Yeong-Jin
Kim, Eun Soo
Lee, Mi-Young
Kang, Lin-Woo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Cannabis sativa L.
has been utilized for a long time as a traditional herbal medicine in Korea.
Dry fruits, achenes, each containing a single seed of Cannabis, are currently prescribed as Ma In (Cannabis Semen), a laxative.
As each achene is enclosed by a bract, in which tetrahydrocannabinol (THC), the main psychological active compound in Cannabis is synthesized; achene is easily contaminated by THC from bract remnants.
Therefore, it is safer to harvest achenes from Cannabis with a low THC content.
Seeds of hemp, a low THC Cannabis, were recently classified as possible sources of new pharmacologically active compounds.
Thus, a proper method to select appropriate Cannabis plants with low THC among cultivars in South Korea for medicinal purpose is necessary.
As a result of cross-selection, Cannabis L.
cultivar “Cheungsam” (CH) with the lowest THC content among cultivars cultivated in South Korea has been developed.
In this study, we developed two DNA markers to reliably discriminate CH from other local cultivars with higher THC contents.
We developed primer sets CHF3/CHR2 to amplify the 642 bp DNA marker of CH based on differences in the nucleotide sequences of the THCA synthase gene, which encodes a key enzyme in THC synthesis.
We then developed a CHF1/CHR3 primer set to amplify the 401 bp DNA marker of CH based on the differences in both the content of very long chain fatty acids (VLCFs) and the sequence of the putative 3-ketoacyl-CoA synthase (KCS) gene encoding enzymes synthesizing VLCFs among local cultivars.
American Psychological Association (APA)
Doh, Eui-Jeong& Lee, Guem-San& Yun, Yeong-Jin& Kang, Lin-Woo& Kim, Eun Soo& Lee, Mi-Young…[et al.]. 2019. DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151113
Modern Language Association (MLA)
Doh, Eui-Jeong…[et al.]. DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151113
American Medical Association (AMA)
Doh, Eui-Jeong& Lee, Guem-San& Yun, Yeong-Jin& Kang, Lin-Woo& Kim, Eun Soo& Lee, Mi-Young…[et al.]. DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151113
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151113