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miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP+-Intoxicated SH-SY5Y Cells
Joint Authors
Zhang, Jianlei
Liu, Wei
Wang, Yabo
Zhao, Shengnan
Chang, Na
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
miR-135a-5p was reported to play a crucial role in the protective effects of hydrogen sulfide against Parkinson’s disease (PD) by targeting rho-associated protein kinase 2 (ROCK2).
However, the role of another member of miR-135 family (miR-135b) and the underlying mechanism in PD are still unclear.
qRT-PCR and western blot showed that miR-135 was downregulated and glycogen synthase kinase 3β (GSK3β) was upregulated at mRNA and protein levels in MPP+-intoxicated SH-SY5Y cells in a dose- and time-dependent manner.
MTT, TUNEL, and ELISA assays revealed that miR-135b overexpression significantly promoted cell proliferation and inhibited apoptosis and production of TNF-α and IL-1β in SH-SY5Y cells in the presence of MPP+.
Luciferase reporter assay demonstrated that GSK3β was a direct target of miR-135b.
Moreover, sodium nitroprusside (SNP), a GSK3β activator, dramatically reversed the effects of miR-135b upregulation on cell proliferation, apoptosis, and inflammatory cytokine production in MPP+-intoxicated SH-SY5Y cells.
Taken together, miR-135b exerts a protective role via promotion of proliferation and suppression of apoptosis and neuroinflammation by targeting GSK3β in MPP+-intoxicated SH-SY5Y cells, providing a potential therapeutic target for the treatment of PD.
American Psychological Association (APA)
Zhang, Jianlei& Liu, Wei& Wang, Yabo& Zhao, Shengnan& Chang, Na. 2017. miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP+-Intoxicated SH-SY5Y Cells. Disease Markers،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1152401
Modern Language Association (MLA)
Zhang, Jianlei…[et al.]. miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP+-Intoxicated SH-SY5Y Cells. Disease Markers No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1152401
American Medical Association (AMA)
Zhang, Jianlei& Liu, Wei& Wang, Yabo& Zhao, Shengnan& Chang, Na. miR-135b Plays a Neuroprotective Role by Targeting GSK3β in MPP+-Intoxicated SH-SY5Y Cells. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1152401
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152401