Upregulation of PDZK1 by Calculus Bovis Sativus May Play an Important Role in Restoring Biliary Transport Function in Intrahepatic Cholestasis
Joint Authors
Li, Xiping
Xu, Yanjiao
Zhang, Chengliang
Liu, Dong
Xiang, Dong
Wu, Tao
Feng, Cheng-Yang
He, Wen-Xi
Lei, Kai
Cai, Hong-Jiao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Intrahepatic cholestasis is a main cause of hepatic accumulation of bile acids leading to liver injury, fibrosis, and liver failure.
Our previous studies proved that Calculus Bovis Sativus (CBS) can restore biliary transport function through upregulating the multidrug resistance-associated protein 2 (MRP2) and breast cancer resistance protein (BCRP) in 17α-ethynylestradiol- (EE-) induced intrahepatic cholestasis rats.
The regulation mechanism of CBS on these transporters, however, remains unclear.
This study was designed to evaluate the possible relationship between the effect of CBS on transport activities and the regulation of CBS on the expression of PDZK1, a mainly scaffold protein which can regulate MRP2 and BCRP.
Intrahepatic cholestasis model was induced in rats with injection of EE for five consecutive days and then the biliary excretion rates and cumulative biliary excretions were measured.
The mRNA and protein expression levels of PDZK1 were detected by reverse transcription-quantitative real-time polymerase chain reaction, western blot, and immunohistochemical analysis.
When treated with CBS, cumulative biliary excretions and mRNA and protein expressions of PDZK1 were significantly increased in intrahepatic cholestasis rats.
This study demonstrated that CBS exerted a beneficial effect on EE-induced intrahepatic cholestasis rats by restoring biliary transport function, which may result from the upregulation of PDZK1 expression.
American Psychological Association (APA)
Xiang, Dong& Wu, Tao& Feng, Cheng-Yang& Li, Xiping& Xu, Yanjiao& He, Wen-Xi…[et al.]. 2017. Upregulation of PDZK1 by Calculus Bovis Sativus May Play an Important Role in Restoring Biliary Transport Function in Intrahepatic Cholestasis. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1153050
Modern Language Association (MLA)
Xiang, Dong…[et al.]. Upregulation of PDZK1 by Calculus Bovis Sativus May Play an Important Role in Restoring Biliary Transport Function in Intrahepatic Cholestasis. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1153050
American Medical Association (AMA)
Xiang, Dong& Wu, Tao& Feng, Cheng-Yang& Li, Xiping& Xu, Yanjiao& He, Wen-Xi…[et al.]. Upregulation of PDZK1 by Calculus Bovis Sativus May Play an Important Role in Restoring Biliary Transport Function in Intrahepatic Cholestasis. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1153050
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153050