Effect of Gelsemium elegans and Mussaenda pubescens, the Components of a Detoxification Herbal Formula, on Disturbance of the Intestinal Absorptions of Indole Alkaloids in Caco-2 Cells
Joint Authors
Wang, Yinghao
Wang, Heshan
Wu, Shuisheng
Li, Desen
Chen, Sainan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Gelsemium elegans (GE) is a kind of well-known toxic plant.
It can be detoxified by Mussaenda pubescens (MP), but the detoxification mechanism is still unclear.
Thus, a detoxification herbal formula (GM) comprising GE and MP was derived.
The Caco-2 cells monolayer model was used to evaluate GM effects on transporting six kinds of indole alkaloids of GE.
The bidirectional transport studies demonstrated that absorbance percentage of indole alkaloids in GE increased linearly over time.
But in GM, Papp (AP→BL) values of the most toxic members, gelsenicine, humantenidine, and gelsevirine, were lower than that of Papp (BL→AP) (P<0.05).
The prominent analgesic effect members, gelsemine and koumine, were approximately 1.00 in γ values.
Nowhere was this increasing efflux more pronounced than in the case of indole alkaloids with N-O structure.
In the presence of verapamil, the γ values of humantenidine, gelsenicine, gelsevirine, and humantenine were decreased by 43.69, 41.42, 36.00, and 8.90 percent, respectively.
The γ values in presence of ciclosporin were homologous with a decrease of 42.32, 40.59, 34.00, and 15.07 percent.
It suggested that the efflux transport was affected by transporters.
Taken together, due to the efflux transporters participation, the increasing efflux of indole alkaloids from GM was found in Caco-2 cells.
American Psychological Association (APA)
Wang, Yinghao& Wang, Heshan& Wu, Shuisheng& Li, Desen& Chen, Sainan. 2017. Effect of Gelsemium elegans and Mussaenda pubescens, the Components of a Detoxification Herbal Formula, on Disturbance of the Intestinal Absorptions of Indole Alkaloids in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154418
Modern Language Association (MLA)
Wang, Yinghao…[et al.]. Effect of Gelsemium elegans and Mussaenda pubescens, the Components of a Detoxification Herbal Formula, on Disturbance of the Intestinal Absorptions of Indole Alkaloids in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1154418
American Medical Association (AMA)
Wang, Yinghao& Wang, Heshan& Wu, Shuisheng& Li, Desen& Chen, Sainan. Effect of Gelsemium elegans and Mussaenda pubescens, the Components of a Detoxification Herbal Formula, on Disturbance of the Intestinal Absorptions of Indole Alkaloids in Caco-2 Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1154418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154418