Evaluation of Anti-Inflammatory Components of Guizhi Fuling Capsule, an Ancient Chinese Herbal Formula, in Human Umbilical Vein Endothelial Cells
Joint Authors
Li, Ping
Gong, Guowei
Zheng, Yuzhong
Xin, Guizhong
Dong, Tina TX
Tsim, Karl W. K.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Guizhi Fuling capsule (GFC), a well-known formula composed of five medicinal herbs, is commonly prescribed to treat primary dysmenorrhea, as well as to achieve good clinical efficacy in China.
However, the active components of GFC have not been identified.
Here, the anti-inflammatory functions of GFC, as well as its major ingredients, were evaluated in human umbilical vein endothelial cells (HUVECs).
Methods.
Lipopolysaccharide (LPS) was used in HUVECs to imitate the cellular inflammation.
Then, GFC-triggered mRNA expressions of cyclooxygenase-1 (COX-1) and COX-2 were determined by real-time PCR, while the expression of COX-2 protein was revealed by western blotting.
Besides, nine components of GFC were evaluated for their contribution value in the anti-dysmenorrhea effects Results.
The application of GFC downregulated the mRNA expressions of COX-1 and COX-2 mRNAs.
Nine major components of GFC were tested in the inflammatory system, and three compounds, including paeoniflorin, benzoylpaeoniflorin, and amygdalin, exhibited robust activation in HUVECs.
The combination of paeoniflorin, benzoylpaeoniflorin, and amygdalin showed over 80% of the anti-inflammatory activation.
Conclusion.
Our study supports that GFC plays a promising role in anti-dysmenorrhea function by decreasing COXs’ expression.
Besides, paeoniflorin, benzoylpaeoniflorin, and amygdalin could be considered as major regulators for the anti-dysmenorrhea effects of GFC.
American Psychological Association (APA)
Zheng, Yuzhong& Xin, Guizhong& Gong, Guowei& Dong, Tina TX& Li, Ping& Tsim, Karl W. K.. 2020. Evaluation of Anti-Inflammatory Components of Guizhi Fuling Capsule, an Ancient Chinese Herbal Formula, in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155047
Modern Language Association (MLA)
Zheng, Yuzhong…[et al.]. Evaluation of Anti-Inflammatory Components of Guizhi Fuling Capsule, an Ancient Chinese Herbal Formula, in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1155047
American Medical Association (AMA)
Zheng, Yuzhong& Xin, Guizhong& Gong, Guowei& Dong, Tina TX& Li, Ping& Tsim, Karl W. K.. Evaluation of Anti-Inflammatory Components of Guizhi Fuling Capsule, an Ancient Chinese Herbal Formula, in Human Umbilical Vein Endothelial Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155047
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155047