Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts
Joint Authors
Sakurai, Takuya
Shirato, Ken
Ohno, Hideki
Koda, Tomoko
Takanari, Jun
Imaizumi, Kazuhiko
Ogasawara, Junetsu
Kizaki, Takako
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ultraviolet (UV) irradiation induces proinflammatory responses in skin cells, including dermal fibroblasts, accelerating premature skin aging (photoaging).
ETAS 50, a standardized extract from the Asparagus officinalis stem, is a novel and unique functional food that suppresses proinflammatory responses of hydrogen peroxide-stimulated skin fibroblasts and interleukin- (IL-) 1β-stimulated hepatocytes.
To elucidate its antiphotoaging potencies, we examined whether ETAS 50 treatment after UV-B irradiation attenuates proinflammatory responses of normal human dermal fibroblasts (NHDFs).
UV-B-irradiated NHDFs showed reduced levels of the cytosolic inhibitor of nuclear factor-κB α (IκBα) protein and increased levels of nuclear p65 protein.
The nuclear factor-κB nuclear translocation inhibitor JSH-23 abolished UV-B irradiation-induced IL-1β mRNA expression, indicating that p65 regulates transcriptional induction.
ETAS 50 also markedly suppressed UV-B irradiation-induced increases in IL-1β mRNA levels.
Immunofluorescence analysis revealed that ETAS 50 retained p65 in the cytosol after UV-B irradiation.
Western blotting also showed that ETAS 50 suppressed the UV-B irradiation-induced increases in nuclear p65 protein.
Moreover, ETAS 50 clearly suppressed UV-B irradiation-induced distribution of importin-α protein levels in the nucleus without recovering cytosolic IκBα protein levels.
These results suggest that ETAS 50 exerts anti-inflammatory effects on UV-B-irradiated NHDFs by suppressing the nuclear import machinery of p65.
Therefore, ETAS 50 may prevent photoaging by suppressing UV irradiation-induced proinflammatory responses of dermal fibroblasts.
American Psychological Association (APA)
Shirato, Ken& Koda, Tomoko& Takanari, Jun& Sakurai, Takuya& Ogasawara, Junetsu& Imaizumi, Kazuhiko…[et al.]. 2018. Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1155403
Modern Language Association (MLA)
Shirato, Ken…[et al.]. Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1155403
American Medical Association (AMA)
Shirato, Ken& Koda, Tomoko& Takanari, Jun& Sakurai, Takuya& Ogasawara, Junetsu& Imaizumi, Kazuhiko…[et al.]. Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1155403
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155403