Luteolin Protects Pheochromocytoma (PC-12)‎ Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway

Joint Authors

Wang, Han-Rui
Pei, Si-Ying
Fan, Dong-Xu
Liu, Yan-Hui
Pan, Xiao-Feng
Song, Fan-Xu
Deng, Shu-Hua
Qiu, Hong-Bin
Zhang, Ning

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-01

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

The regulatory effect of luteolin on the progression of Alzheimer’s disease (AD) remains unclear from the perspective of apoptosis.

The present study aimed to investigate the protective effects of luteolin against Aβ25-35-induced cell apoptosis in pheochromocytoma (PC-12) cells.

Aβ25-35 was used to induce an in vitro model of AD.

Estradiol was used as a positive control.

The PC-12 cells were incubated with luteolin alone or in combination with fulvestrant or U0126.

The results showed that luteolin treatment significantly prevents Aβ25-35-induced decrease in cell viability and inhibits Aβ25-35-induced cell apoptosis.

After the addition of fulvestrant and U0126, the apoptosis rate of PC-12 cells increased significantly.

In addition, luteolin treatment significantly upregulated the expression of Bcl-2 and downregulated the expression of Bax and caspase-3, whereas fulvestrant and U0126 partially reversed the effects of luteolin.

Moreover, luteolin treatment upregulated the expression of ERβ and p-ERK1/2, whereas fulvestrant blocked the expression of p-ERK1/2.

The study showed that luteolin could activate the ER/ERK/MAPK signalling pathway to protect PC-12 cells against Aβ25-35-induced cell apoptosis via selectively acting on ERβ.

Thus, luteolin may be considered as a potential novel therapeutic strategy for AD.

American Psychological Association (APA)

Wang, Han-Rui& Pei, Si-Ying& Fan, Dong-Xu& Liu, Yan-Hui& Pan, Xiao-Feng& Song, Fan-Xu…[et al.]. 2020. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155448

Modern Language Association (MLA)

Wang, Han-Rui…[et al.]. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1155448

American Medical Association (AMA)

Wang, Han-Rui& Pei, Si-Ying& Fan, Dong-Xu& Liu, Yan-Hui& Pan, Xiao-Feng& Song, Fan-Xu…[et al.]. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155448

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1155448