Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway
Joint Authors
Wang, Han-Rui
Pei, Si-Ying
Fan, Dong-Xu
Liu, Yan-Hui
Pan, Xiao-Feng
Song, Fan-Xu
Deng, Shu-Hua
Qiu, Hong-Bin
Zhang, Ning
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The regulatory effect of luteolin on the progression of Alzheimer’s disease (AD) remains unclear from the perspective of apoptosis.
The present study aimed to investigate the protective effects of luteolin against Aβ25-35-induced cell apoptosis in pheochromocytoma (PC-12) cells.
Aβ25-35 was used to induce an in vitro model of AD.
Estradiol was used as a positive control.
The PC-12 cells were incubated with luteolin alone or in combination with fulvestrant or U0126.
The results showed that luteolin treatment significantly prevents Aβ25-35-induced decrease in cell viability and inhibits Aβ25-35-induced cell apoptosis.
After the addition of fulvestrant and U0126, the apoptosis rate of PC-12 cells increased significantly.
In addition, luteolin treatment significantly upregulated the expression of Bcl-2 and downregulated the expression of Bax and caspase-3, whereas fulvestrant and U0126 partially reversed the effects of luteolin.
Moreover, luteolin treatment upregulated the expression of ERβ and p-ERK1/2, whereas fulvestrant blocked the expression of p-ERK1/2.
The study showed that luteolin could activate the ER/ERK/MAPK signalling pathway to protect PC-12 cells against Aβ25-35-induced cell apoptosis via selectively acting on ERβ.
Thus, luteolin may be considered as a potential novel therapeutic strategy for AD.
American Psychological Association (APA)
Wang, Han-Rui& Pei, Si-Ying& Fan, Dong-Xu& Liu, Yan-Hui& Pan, Xiao-Feng& Song, Fan-Xu…[et al.]. 2020. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155448
Modern Language Association (MLA)
Wang, Han-Rui…[et al.]. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1155448
American Medical Association (AMA)
Wang, Han-Rui& Pei, Si-Ying& Fan, Dong-Xu& Liu, Yan-Hui& Pan, Xiao-Feng& Song, Fan-Xu…[et al.]. Luteolin Protects Pheochromocytoma (PC-12) Cells against Aβ25-35-Induced Cell Apoptosis through the ERERKMAPK Signalling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1155448
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155448