α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPTNAD Controlled Inflammatory Reactions
Joint Authors
Li, Yan
Dong, Jiyang
Zuo, Jian
Tao, Mengqing
Jiang, Jia
Wang, Lin
Mao, Qingcheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
α-Mangostin (MAN) is a bioactive xanthone isolated from mangosteen.
This study was designed to investigate its therapeutic effects on acute lung injury (ALI) and explore the underlying mechanisms of action.
Rats from treatment groups were subject to oral administration of MAN for 3 consecutive days beforehand, and then ALI was induced in all the rats except for normal controls via an intraperitoneal injection with lipopolysaccharide.
The severity of disease was evaluated by histological examination and hematological analysis.
Protein expressions in tissues and cells were examined with immunohistochemical and immunoblotting methods, respectively.
The levels of cytokines and nicotinamide adenine dinucleotide (NAD) were determined using ELISA and colorimetric kits, respectively.
It was found that MAN treatment significantly improved histological conditions, reduced leucocytes counts, relieved oxidative stress, and declined TNF-α levels in ALI rats.
Meanwhile, MAN treatment decreased expressions of nicotinamide phosphoribosyltransferase (NAMPT) and Sirt1 both in vivo and in vitro, which was accompanied with a synchronized decline of NAD and TNF-α.
Immunoblotting assay further showed that MAN downregulated HMGB1, TLR4, and p-p65 in RAW 264.7 cells.
MAN induced declines of both HMGB1/TLR4/p-p65 and TNF-α were substantially reversed by cotreatment with nicotinamide mononucleotide or NAD.
These results suggest that downregulation of NAMPT/NAD by MAN treatments contributes to the alleviation of TLR4/NF-κB-mediated inflammations in macrophage, which is essential for amelioration of ALI in rats.
American Psychological Association (APA)
Tao, Mengqing& Jiang, Jia& Wang, Lin& Li, Yan& Mao, Qingcheng& Dong, Jiyang…[et al.]. 2018. α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPTNAD Controlled Inflammatory Reactions. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155538
Modern Language Association (MLA)
Tao, Mengqing…[et al.]. α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPTNAD Controlled Inflammatory Reactions. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1155538
American Medical Association (AMA)
Tao, Mengqing& Jiang, Jia& Wang, Lin& Li, Yan& Mao, Qingcheng& Dong, Jiyang…[et al.]. α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPTNAD Controlled Inflammatory Reactions. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155538
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155538