A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva

Joint Authors

Alvi, Syed N.
Hammami, Muhammad M.

Source

International Journal of Analytical Chemistry

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-03-03

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Chemistry
Science

Abstract EN

A simple ultraperformance liquid chromatography-tandem mass spectrometry assay for measurement of cortisol level in human saliva was developed and validated.

Saliva samples containing cortisol were spiked with tolperisone as internal standard (IS) and extracted with a mixture of methyl tert-butyl ether and hexane (8:2, v:v).

After solvent evaporation, residue was reconstituted in 100 μl mobile phase.

Analysis was performed on Atlantis dC18 column (2.1 × 100 mm, 3 μm particle size) with a mobile phase composed of acetonitrile and 2 mM ammonium acetate (50:50, v:v) and delivered at a flow rate of 0.3 ml/minute.

Mass spectrometry acquisition was performed with multiple reaction monitoring in positive-ion mode for cortisol and IS (m/z: 363.1 → 121.0 and 246.0 → 97.9, respectively).

Retention times of cortisol and IS were about 1.35 and 2.45 minutes, respectively.

The relationship between cortisol level and peak area ratio of cortisol to IS was linear in the range of 0.5-100 ng/ml.

Intra- and interday coefficient of variation and bias were ≤ 9.0% and ≤12.0%, respectively.

Mean extraction recoveries of cortisol and IS from saliva samples were 92% and 94%, respectively.

Using the method, cortisol was found to be ≥ 86% stable in processed (24 hours at room temperature or 48 hours at -20°C) and ≥ 91% stable in unprocessed (24 hours at room temperature or 20 weeks at -20°C) saliva samples.

Further, the method was successfully applied to determine daily cortisol profile in saliva samples of a healthy volunteer.

American Psychological Association (APA)

Alvi, Syed N.& Hammami, Muhammad M.. 2019. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1155899

Modern Language Association (MLA)

Alvi, Syed N.& Hammami, Muhammad M.. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1155899

American Medical Association (AMA)

Alvi, Syed N.& Hammami, Muhammad M.. A Simple Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Method for Measurement of Cortisol Level in Human Saliva. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1155899

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1155899