Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics
Joint Authors
Li, Qiyan
Zhu, Riran
Li, Jun
Wang, Xiaobing
Xu, Lihua
Li, Yanshen
Li, Peng
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed.
The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF).
The limit of detection (LOD), measured by IC10, was 0.0021 ng mL−1.
The detection range (IC20-IC80) was ranged from 0.00979 to 0.12026 ng mL−1.
In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively.
Moreover, with the help of HRP-labeled anti-CAP mAb, the method could be processed in fast direct immunoreaction mode.
This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination.
American Psychological Association (APA)
Li, Qiyan& Zhu, Riran& Li, Jun& Wang, Xiaobing& Xu, Lihua& Li, Yanshen…[et al.]. 2019. Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1155952
Modern Language Association (MLA)
Li, Qiyan…[et al.]. Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1155952
American Medical Association (AMA)
Li, Qiyan& Zhu, Riran& Li, Jun& Wang, Xiaobing& Xu, Lihua& Li, Yanshen…[et al.]. Highly Specific Chemiluminescence Immunoassay for the Determination of Chloramphenicol in Cosmetics. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1155952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155952