Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells
Joint Authors
Zaidan Dagli, Maria Lúcia
Alves da Paz, Diego Pinha
Nagamine, Márcia Kazumi
Grande, Murilo Penteado Del
Leite, João Vitor Pereira
Sobreira, Flavia Mendonça Gonçalves
Bacchi, Elfriede M.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-14
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Euphorbia tirucalli Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant.
E.
tirucalli produces a latex that is commonly used to treat neoplasms.
This study aimed to evaluate the effects of diluted E.
tirucalli latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells.
SK-MEL-28 (3 × 103 cells/well) and CBMY (6 × 103 cells/well) were cultivated in 96-well plates.
The cells were treated with 50 μl/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the E.
tirucalli latex (ETL) in DMEM.
Control group cells received 50 μl/well of DMEM.
After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay.
There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL.
Human melanoma cells presented a typical U shape curve, characteristic of hormesis.
To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells.
Therefore, DETL is a potentially new antineoplastic drug.
American Psychological Association (APA)
Alves da Paz, Diego Pinha& Nagamine, Márcia Kazumi& Grande, Murilo Penteado Del& Leite, João Vitor Pereira& Sobreira, Flavia Mendonça Gonçalves& Bacchi, Elfriede M.…[et al.]. 2020. Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1156049
Modern Language Association (MLA)
Alves da Paz, Diego Pinha…[et al.]. Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-5.
https://search.emarefa.net/detail/BIM-1156049
American Medical Association (AMA)
Alves da Paz, Diego Pinha& Nagamine, Márcia Kazumi& Grande, Murilo Penteado Del& Leite, João Vitor Pereira& Sobreira, Flavia Mendonça Gonçalves& Bacchi, Elfriede M.…[et al.]. Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-5.
https://search.emarefa.net/detail/BIM-1156049
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156049