Cafestol Inhibits High-Glucose-Induced Cardiac Fibrosis in Cardiac Fibroblasts and Type 1-Like Diabetic Rats
Joint Authors
Liu, Ju-Chi
Hao, Wen-Rui
Liu, Yi-Chung
Hong, Hong-Jye
Chen, Po-Yuan
Lyu, Ping-Chiang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Diabetes is associated with the development of myocardial fibrosis, which is related to various cardiac diseases.
Cafestol, one of the active ingredients in coffee, has been reported to exert biological effects.
However, whether cafestol can ameliorate diabetes-induced cardiac fibrosis remains unknown.
The aim of this study was to evaluate the effects of cafestol on cardiac fibrosis in high-glucose-treated cardiac fibroblasts and streptozocin- (STZ-) induced diabetic rats.
Rat cardiac fibroblasts were cultured in high-glucose (25 mM) media in the absence or presence of cafestol, and the changes in collagen synthesis, transforming growth factor-β1 (TGF-β1) production, and related signaling molecules were assessed on the basis of 3H-proline incorporation, enzyme-linked immunosorbent assay, and western blotting.
Cardiac fibroblasts exposed to high-glucose conditions exhibited increased collagen synthesis, TGF-β1 production, and Smad2/3 phosphorylation, and these effects were mitigated by cafestol treatment.
Furthermore, cafestol increased the translocation of nuclear factor erythroid 2-related factor 2 and increased the expression of heme oxygenase-1.
The results of molecular docking analysis suggested a selective interaction of cafestol with Kelch-like ECH-associated protein 1.
The rats with untreated STZ-induced diabetes exhibited considerable collagen accumulation, which was ameliorated by cafestol.
Moreover, activities of catalase, superoxide dismutase, general matrix metalloproteinase, and reduced glutathione concentration were upregulated, whereas malondialdehyde level was downregulated by treatment with cafestol in rats with cardiac fibrosis.
These findings highlight the effects of cafestol, which may be useful in treating diabetes-related cardiac fibrosis.
American Psychological Association (APA)
Liu, Ju-Chi& Chen, Po-Yuan& Hao, Wen-Rui& Liu, Yi-Chung& Lyu, Ping-Chiang& Hong, Hong-Jye. 2020. Cafestol Inhibits High-Glucose-Induced Cardiac Fibrosis in Cardiac Fibroblasts and Type 1-Like Diabetic Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156157
Modern Language Association (MLA)
Liu, Ju-Chi…[et al.]. Cafestol Inhibits High-Glucose-Induced Cardiac Fibrosis in Cardiac Fibroblasts and Type 1-Like Diabetic Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1156157
American Medical Association (AMA)
Liu, Ju-Chi& Chen, Po-Yuan& Hao, Wen-Rui& Liu, Yi-Chung& Lyu, Ping-Chiang& Hong, Hong-Jye. Cafestol Inhibits High-Glucose-Induced Cardiac Fibrosis in Cardiac Fibroblasts and Type 1-Like Diabetic Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156157