Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven
Joint Authors
Zavala-Sánchez, Miguel A.
Zamilpa, Alejandro
Tortoriello, Jaime
Herrera-Arellano, Armando
Morales, Dulce
Ramirez, Guillermo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Current antiobesity and antidiabetic tools have been insufficient to curb these diseases and frequently cause side effects; therefore, new pancreatic lipase and α–glucosidase inhibitors could be excellent aids for the prevention and treatment of these diseases.
The aim of this study was to identify, quantify, and characterize the chemical compounds with the highest degree of inhibitory activity of these enzymes, contained in a Ludwigia octovalvis hydroalcoholic extract.
Chemical purification was performed by liquid–liquid separation and column chromatography.
Inhibitory activities were measured in vitro, employing acarbose, orlistat, and a Camellia sinensis hydroalcoholic extract as references.
For structural elucidation, Nuclear Magnetic Resonance was carried out, and High Performance Liquid Chromatography was used to quantify the compounds.
For α–glucosidases, L.
octovalvis hydroalcoholic extract and its ethyl acetate fraction showed half–maximal Inhibitory Concentration (IC50) values of 700 and 250 μg/mL, for lipase, 480 and 718 μg/mL, while C.
sinensis showed 260 and 587 μg/mL.
The most active compounds were identified as ethyl gallate (1, IC50 832 μM) and gallic acid (2, IC50 969 μM); both displayed competitive inhibition of α–glucosidases and isoorientin (3, IC50 201 μM), which displayed uncompetitive inhibition of lipase.
These data could be useful in the development of a novel phytopharmaceutical drug.
American Psychological Association (APA)
Morales, Dulce& Ramirez, Guillermo& Herrera-Arellano, Armando& Tortoriello, Jaime& Zavala-Sánchez, Miguel A.& Zamilpa, Alejandro. 2018. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
Modern Language Association (MLA)
Morales, Dulce…[et al.]. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
American Medical Association (AMA)
Morales, Dulce& Ramirez, Guillermo& Herrera-Arellano, Armando& Tortoriello, Jaime& Zavala-Sánchez, Miguel A.& Zamilpa, Alejandro. Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156726
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156726