Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD
Joint Authors
Silva, Maria Goretti de Vasconcelos
Lima, Leandro Bezerra
Oliveira, Maria da Conceição Ferreira de
Mattos, Marcos Carlos de
Mafezoli, Jair
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-03
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The concentration of diterpenes barbatusin (1) and 3β-hydroxy-3-deoxybarbatusin (2) in the extracts from leaves of Plectranthus grandis, P.
barbatus, P.
ornatus, and P.
amboinicus was evaluated by HPLC-DAD analysis on a Luna C-18 column, using isocratic mixtures of water and acetonitrile as eluents.
The regression equations were obtained with good linearity (r2 > 0.99) and limit of quantifications was higher than 0.1 µg/mL.
The precision (lower than 3.5%, within day) and accuracy (higher than 81.7% and lower than 107.6%) of the methods were adequate.
Barbatusin (1) was detected in P.
grandis (15.432 ± 2.28 mg/g) and P.
barbatus (5.198 ± 3.45 mg/g) extracts, while compound 2 was detected in P.
grandis (4.068 ± 3.34 mg/g), P.
barbatus (0.654 ± 5.86 mg/g), P.
amboinicus (0.160 ± 7.25 mg/g), and P.
ornatus (0.763 ± 5.10 mg/g).
The evaluated validation parameters were satisfactorily achieved, and the developed methodology represents a suitable tool for application in the quantification of barbatusin (1) and 3β-hydroxy-3-deoxybarbatusin (2) in Plectranthus species.
American Psychological Association (APA)
Silva, Maria Goretti de Vasconcelos& Lima, Leandro Bezerra& Oliveira, Maria da Conceição Ferreira de& Mattos, Marcos Carlos de& Mafezoli, Jair. 2017. Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1157511
Modern Language Association (MLA)
Silva, Maria Goretti de Vasconcelos…[et al.]. Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1157511
American Medical Association (AMA)
Silva, Maria Goretti de Vasconcelos& Lima, Leandro Bezerra& Oliveira, Maria da Conceição Ferreira de& Mattos, Marcos Carlos de& Mafezoli, Jair. Quantification of Barbatusin and 3β-Hydroxy-3-deoxybarbatusin in Plectranthus Species by HPLC-DAD. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1157511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157511