Effects of sEA on Slow Transit Constipation through the Microbiota-Gut-Brain Axis in Rats
Joint Authors
Jin, Xun
Guan, Yanting
Bai, Hua
Liu, Yan
Lv, Xing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
To investigate the effect of sacral electroacupuncture (sEA) on the microbiota-gut-brain axis in the treatment of slow transit constipation, this study established a drug-induced model of slow transit constipation in rats and carried out sEA at the Baliao acupoints (BL31-BL34).
On the 14th day of the therapeutic period (24 h fecal pellets), the aquaporin 3 (AQP3), 5-hydroxytryptamine (5-HT), and substance P (SP) transcripts from the distal colon and hypothalamus were analyzed.
16S rDNA has been widely used to analyze the diversity of the microbial communities.
Therefore, in the present study, changes in the intestinal microbiota were analyzed by 16S rDNA gene sequencing.
The results showed that sEA significantly increased the number of fecal pellets and the water content in the feces and reduced the reabsorption of intestinal water in 24 h.
sEA also upregulated the level of SP mRNA expression in the distal colon and the hypothalamus, but downregulated the level of 5-HT mRNA expression in the distal colon.
Moreover, sEA improved the Bacteroidetes to Firmicutes (B/F) ratio, which is beneficial to the general structure of the intestinal microflora.
Our findings suggested that the microbiota-gut-brain axis constitutes a crucial pathological basis in the development of slow transit constipation.
sEA improved the slow transit constipation by regulating the balance of the microbiota-gut-brain axis.
American Psychological Association (APA)
Jin, Xun& Guan, Yanting& Bai, Hua& Liu, Yan& Lv, Xing. 2020. Effects of sEA on Slow Transit Constipation through the Microbiota-Gut-Brain Axis in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158015
Modern Language Association (MLA)
Jin, Xun…[et al.]. Effects of sEA on Slow Transit Constipation through the Microbiota-Gut-Brain Axis in Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1158015
American Medical Association (AMA)
Jin, Xun& Guan, Yanting& Bai, Hua& Liu, Yan& Lv, Xing. Effects of sEA on Slow Transit Constipation through the Microbiota-Gut-Brain Axis in Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1158015
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158015