Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro
Joint Authors
T-Johari, Syed Ahmad Tajudin
Hashim, Fatimah
Ismail, Wan Iryani Wan
Ali, Abdul Manaf
Source
International Journal of Cell Biology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Combination of natural products with chemodrugs is becoming a trend in discovering new therapeutics approach for enhancing the cancer treatment process.
In the present study, we aimed to investigate the cytotoxic and apoptosis induction of Gelam honey (GH) combined with or without 5-Fluorouracil (5-FU) on HT-29 cells.
The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to assess cytotoxicity.
Morphological changes and apoptosis were determined by the inverted microscope, Annexin V-FITC, and DNA fragmentation via flow cytometric analysis, respectively.
Our results demonstrate that combined treatment revealed a remarkable and concentration-dependent cytotoxic effect on HT-29 cells in comparison with GH and 5-FU alone.
Flow cytometry analysis showed that early apoptosis event was more pronounced in combined treatment.
In addition, compared to 5-FU alone, apoptosis of HT-29 cells treated with combinations of GH and 5-FU demonstrated increasing percentages of fragmented DNA.
Our results suggest that GH has a synergistic cytotoxic effect with 5-FU in HT-29 cell lines in vitro.
Although the actions of the molecular mechanisms are not yet clear, the results reveal that the combination of GH and 5-FU could have the potential as a therapeutic agent.
American Psychological Association (APA)
T-Johari, Syed Ahmad Tajudin& Hashim, Fatimah& Ismail, Wan Iryani Wan& Ali, Abdul Manaf. 2019. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1158530
Modern Language Association (MLA)
T-Johari, Syed Ahmad Tajudin…[et al.]. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1158530
American Medical Association (AMA)
T-Johari, Syed Ahmad Tajudin& Hashim, Fatimah& Ismail, Wan Iryani Wan& Ali, Abdul Manaf. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1158530
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158530