Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro

Joint Authors

T-Johari, Syed Ahmad Tajudin
Hashim, Fatimah
Ismail, Wan Iryani Wan
Ali, Abdul Manaf

Source

International Journal of Cell Biology

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-02-21

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

Combination of natural products with chemodrugs is becoming a trend in discovering new therapeutics approach for enhancing the cancer treatment process.

In the present study, we aimed to investigate the cytotoxic and apoptosis induction of Gelam honey (GH) combined with or without 5-Fluorouracil (5-FU) on HT-29 cells.

The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to assess cytotoxicity.

Morphological changes and apoptosis were determined by the inverted microscope, Annexin V-FITC, and DNA fragmentation via flow cytometric analysis, respectively.

Our results demonstrate that combined treatment revealed a remarkable and concentration-dependent cytotoxic effect on HT-29 cells in comparison with GH and 5-FU alone.

Flow cytometry analysis showed that early apoptosis event was more pronounced in combined treatment.

In addition, compared to 5-FU alone, apoptosis of HT-29 cells treated with combinations of GH and 5-FU demonstrated increasing percentages of fragmented DNA.

Our results suggest that GH has a synergistic cytotoxic effect with 5-FU in HT-29 cell lines in vitro.

Although the actions of the molecular mechanisms are not yet clear, the results reveal that the combination of GH and 5-FU could have the potential as a therapeutic agent.

American Psychological Association (APA)

T-Johari, Syed Ahmad Tajudin& Hashim, Fatimah& Ismail, Wan Iryani Wan& Ali, Abdul Manaf. 2019. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1158530

Modern Language Association (MLA)

T-Johari, Syed Ahmad Tajudin…[et al.]. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1158530

American Medical Association (AMA)

T-Johari, Syed Ahmad Tajudin& Hashim, Fatimah& Ismail, Wan Iryani Wan& Ali, Abdul Manaf. Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro. International Journal of Cell Biology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1158530

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1158530