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Regulatory Effect of 1,25(OH)2D3 on TGF-β1 and miR-130b Expression in Streptozotocin-Induced Diabetic Nephropathy in Rats
Joint Authors
Liu, Yuetong
Yang, Ye
Wang, Qin
Kahaer, Apaer
Zhang, Jiyun
Liao, Jing
Abudureyimu, Mairemugu
Yahefu, Reyila
Qi, Jing
Zhao, Lei
Zhu, Jun
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To investigate the role of microRNA-130b in 1,25(OH)2D3 mediated improvement of renal fibrosis via transforming growth factor-beta 1 in a rat model of diabetic nephropathy (DN).
Methods.
DN was induced in 30 rats by intraperitoneal injection of streptozotocin.
These rats were randomly allocated to the DN group, TGF-β1 overexpression group (in situ injection of TGF-β1 lentivirus to kidney tissues), and TGF-β1 siRNA group (in situ injection of TGF-β1 siRNA lentivirus to kidney tissues).
Rats with different expression levels of TGF-β1 were administered 1,25(OH)2D3 (0.03 μg/kg/d) or peanut oil as control.
DN rats were treated only with peanut oil.
All rats were randomly divided into five groups (n = 6 per group): TGF-β1 overexpression + oil, TGF-β1 overexpression + 1,25(OH)2D3, TGF-β1 siRNA + oil, TGF-β1 siRNA + 1,25(OH)2D3, and DN + oil groups.
After 37 days, kidney samples were collected and the expression of TGF-β1 and miR-130b was determined by real-time PCR, western blotting, and immunohistochemistry.
Hematoxylin and eosin staining and Masson staining were used to evaluate kidney morphological and fibrogenic changes.
Differences were determined using ANOVA and Student’s t-test.
Results.
RT-PCR, western blotting, and immunohistochemistry revealed that interference of TGF-β1 significantly decreased mRNA and protein levels of TGF-β1 in renal tissues of DN rats compared to those in renal tissues of rats overexpressing TGF-β1 (p<0.05).
Histological analysis showed that upregulated TGF-β1 led to disorganized kidney structure and severe kidney fibrosis.
The expression of miR-130b was significantly lowered upon lentivirus-mediated overexpression of TGF-β1 than upon downregulation of TGF-β1 (p<0.05).
Treatment with 1,25(OH)2D3 led to a significant reduction of TGF-β1 at the mRNA and protein levels (both p<0.05), improvement of renal structure and fibrosis, and an increase in miR-130b expression (p<0.05).
Conclusion.
TGF-β1 can decrease the expression of miR-130b in kidney tissues of DN rats.
Moreover, miR-130b may be involved in the protective effect of 1,25(OH)2D3 on renal fibrosis via TGF-β1.
American Psychological Association (APA)
Liu, Yuetong& Yang, Ye& Wang, Qin& Kahaer, Apaer& Zhang, Jiyun& Liao, Jing…[et al.]. 2019. Regulatory Effect of 1,25(OH)2D3 on TGF-β1 and miR-130b Expression in Streptozotocin-Induced Diabetic Nephropathy in Rats. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1159315
Modern Language Association (MLA)
Liu, Yuetong…[et al.]. Regulatory Effect of 1,25(OH)2D3 on TGF-β1 and miR-130b Expression in Streptozotocin-Induced Diabetic Nephropathy in Rats. International Journal of Endocrinology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1159315
American Medical Association (AMA)
Liu, Yuetong& Yang, Ye& Wang, Qin& Kahaer, Apaer& Zhang, Jiyun& Liao, Jing…[et al.]. Regulatory Effect of 1,25(OH)2D3 on TGF-β1 and miR-130b Expression in Streptozotocin-Induced Diabetic Nephropathy in Rats. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1159315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159315