Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator
Joint Authors
Morgan, Julie E.
Greer, Susanna F.
Source
International Journal of Cell Biology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The Class II Transactivator (CIITA) is essential to the regulation of Major Histocompatibility Class II (MHC II) genes transcription.
As the “master regulator” of MHC II transcription, CIITA regulation is imperative and requires various posttranslational modifications (PTMs) in order to facilitate its role.
Previously we identified various ubiquitination events on CIITA.
Monoubiquitination is important for CIITA transactivity, while K63 linked ubiquitination is involved in crosstalk with ERK1/2 phosphorylation, where together they mediate cellular movement from the cytoplasm to nuclear region.
Further, CIITA is also modified by degradative K48 polyubiquitination.
However, the E3 ligase responsible for these modifications was unknown.
We show CIITA ubiquitination and transactivity are enhanced with the histone acetyltransferase (HAT), p300/CBP associated factor (pCAF), and the E3 ligase region within pCAF is necessary for both.
Additionally, pCAF mediated ubiquitination is independent of pCAF’s HAT domain, and acetylation deficient CIITA is K48 polyubiquitinated and degraded in the presence of pCAF.
Lastly, we identify the histone acetyltransferase, pCAF, as the E3 ligase responsible for CIITA’s ubiquitination.
American Psychological Association (APA)
Morgan, Julie E.& Greer, Susanna F.. 2017. Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator. International Journal of Cell Biology،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1165146
Modern Language Association (MLA)
Morgan, Julie E.& Greer, Susanna F.. Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator. International Journal of Cell Biology No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1165146
American Medical Association (AMA)
Morgan, Julie E.& Greer, Susanna F.. Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator. International Journal of Cell Biology. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1165146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1165146