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Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii
Joint Authors
Souza, Érica Simplício
Cruz, Kátia Santana
Lima, Emerson Silva
Silva, Marcia de Jesus Amazonas da
Montoia, Andreia
Souza, João Vicente Braga de
Pohlit, Adrian Martin
Source
International Journal of Microbiology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Cryptococcosis is a fungal disease of bad prognosis due to its pathogenicity and the toxicity of the drugs used for its treatment.
The aim of this study was to investigate the medicinal potential of carbazole and β-carboline alkaloids and derivatives against Cryptococcus neoformans and C.
gattii.
Methods.
MICs were established in accordance with the recommendations of the Clinical and Laboratory Standards Institute for alkaloids and derivatives against C.
neoformans and C.
gattii genotypes VNI and VGI, respectively.
A single active compound was further evaluated against C.
neoformans genotypes VNII, VNIII, and VNIV, C.
gattii genotypes VGI, VGIII, and VGIV, Candida albicans ATCC 36232, for cytotoxicity against the MRC-5 lineage of human fibroblasts and for effects on fungal cells (cell wall, ergosterol, and leakage of nucleic acids).
Results.
Screening of 11 compounds revealed 8-nitroharmane as a significant inhibitor (MIC 40 μg/mL) of several C.
neoformans and C.
gattii genotypes.
It was not toxic to fibroblasts (IC50 > 50 µg/mL) nor did it alter fungal cell walls or the concentration of ergosterol in C.
albicans or C.
neoformans.
It increased leakage of substances that absorb at 260 nm.
Conclusions.
The synthetic β-carboline 8-nitroharmane significantly inhibits pathogenic Cryptococcus species and is interesting as a lead compound towards new therapy for Cryptococcus infections.
American Psychological Association (APA)
Cruz, Kátia Santana& Lima, Emerson Silva& Silva, Marcia de Jesus Amazonas da& Souza, Érica Simplício& Montoia, Andreia& Pohlit, Adrian Martin…[et al.]. 2019. Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii. International Journal of Microbiology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1166281
Modern Language Association (MLA)
Cruz, Kátia Santana…[et al.]. Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii. International Journal of Microbiology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1166281
American Medical Association (AMA)
Cruz, Kátia Santana& Lima, Emerson Silva& Silva, Marcia de Jesus Amazonas da& Souza, Érica Simplício& Montoia, Andreia& Pohlit, Adrian Martin…[et al.]. Screening and Antifungal Activity of a β-Carboline Derivative against Cryptococcus neoformans and C. gattii. International Journal of Microbiology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1166281
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166281