Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
Joint Authors
Tong, Chunyi
Liu, Qingxin
Fan, Jialong
Zhou, Chuang
Wang, Liqun
Zhao, Bin
Zhang, Haibin
Liu, Bin
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-02
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer.
With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM.
Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues.
The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy.
In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference.
Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method.
The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA.
American Psychological Association (APA)
Liu, Qingxin& Fan, Jialong& Zhou, Chuang& Wang, Liqun& Zhao, Bin& Zhang, Haibin…[et al.]. 2018. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
Modern Language Association (MLA)
Liu, Qingxin…[et al.]. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
American Medical Association (AMA)
Liu, Qingxin& Fan, Jialong& Zhou, Chuang& Wang, Liqun& Zhao, Bin& Zhang, Haibin…[et al.]. Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1166362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166362