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Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method
Joint Authors
Cordaro, Massimo
Marigo, Luca
Callà, Cinzia
Rossetti, Diana Valeria
Santini, Stefano Angelo
Amalfitano, Adriana
Nocca, Giuseppina
Spagnuolo, Gianrico
Source
International Journal of Analytical Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line.
The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v).
The method was linear in the concentration range of 1.5–120 μg /mL (n = 5).
The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve.
The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively.
The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%.
The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL.
We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.
American Psychological Association (APA)
Nocca, Giuseppina& Callà, Cinzia& Santini, Stefano Angelo& Amalfitano, Adriana& Marigo, Luca& Rossetti, Diana Valeria…[et al.]. 2018. Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method. International Journal of Analytical Chemistry،Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1166432
Modern Language Association (MLA)
Nocca, Giuseppina…[et al.]. Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method. International Journal of Analytical Chemistry No. 2018 (2018), pp.1-5.
https://search.emarefa.net/detail/BIM-1166432
American Medical Association (AMA)
Nocca, Giuseppina& Callà, Cinzia& Santini, Stefano Angelo& Amalfitano, Adriana& Marigo, Luca& Rossetti, Diana Valeria…[et al.]. Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method. International Journal of Analytical Chemistry. 2018. Vol. 2018, no. 2018, pp.1-5.
https://search.emarefa.net/detail/BIM-1166432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166432