Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway
Joint Authors
Xu, Xin
Li, Guoliang
Zhu, Quanli
Ma, Junjian
Pan, Zhijian
Wu, Wei
Source
Gastroenterology Research and Practice
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Somatostatin (SST) has a protective role in intestinal injury, inflammatory response, and intestinal mucosal barrier in rats with acute pancreatitis.
However, its function in sepsis-induced intestinal barrier dysfunction remains largely unknown.
A mouse sepsis model was constructed, and SST was injected into the tail vein.
Then, hematoxylin and eosin staining (HE) was used to detect the intestinal barrier dysfunction.
Enzyme-linked immunosorbent assay was used to detect the level of tumor necrosis factor α- (TNF-) α, interleukin- (IL-) 6, and interleukin- (IL-) 10 in the ileum.
Expressions of tight junction proteins, zonula occludens- (ZO-) 1 and Claudin-1, and NF-κB p65 in the ileum were detected using western blot and immunohistochemistry as needed.
Furthermore, JSH-23 as an inhibitor of the NF-κB pathway was injected into sepsis mice with SST or not.
Mice with sepsis showed an obvious intestinal barrier dysfunction with decreasing specific somatostatin receptor subtype (SSTRs), and increasing TNF-α, IL-6, and IL-10 in the ileum.
SST could relieve the injury, the decrease of SSTRs, and the increase of TNF-α and IL-6 induced by sepsis and also further enhanced the expression of IL-10.
Further analysis showed that ZO-1 and Claudin-1 were reduced in the ileum by sepsis but enhanced by SST.
NF-κB p65 was promoted in the ileum by sepsis but inhibited by SST.
Further experiments confirmed that NF-κB inhibitor JSH-23 could repair the intestinal barrier dysfunction and enhance the protective effect of SST on the intestinal barrier.
SST, with a protective effect on intestinal barrier dysfunction through suppression of NF-κB, could be a potential therapeutic drug for sepsis-induced intestinal barrier dysfunction.
American Psychological Association (APA)
Xu, Xin& Zhu, Quanli& Li, Guoliang& Ma, Junjian& Pan, Zhijian& Wu, Wei. 2020. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
Modern Language Association (MLA)
Xu, Xin…[et al.]. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
American Medical Association (AMA)
Xu, Xin& Zhu, Quanli& Li, Guoliang& Ma, Junjian& Pan, Zhijian& Wu, Wei. Protective Role of Somatostatin in Sepsis-Induced Intestinal Barrier Dysfunction through Inhibiting the Activation of NF-κB Pathway. Gastroenterology Research and Practice. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1166722
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1166722