Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites
Joint Authors
Peng, Gang
Yu, Yadong
Chen, Xiaojun
Huang, He
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Herein, a rapid and highly sensitive amperometric biosensor for the detection of α-ketoglutarate (α-KG) was constructed via an electrochemical approach, in which the glutamate dehydrogenase (GLUD) was modified on the surface of reduced graphene oxide-gold nanoparticle composite (rGO-Aunano composite).
The rGO-Aunano composite was one-step electrodeposited onto glassy carbon electrode (GCE) surface and was characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and electrochemical techniques.
In addition, the rGO-Aunano/GCE was also found to electrocatalyze the oxidation of β-nicotinamide adenine dinucleotide (NADH) at the peak potential of 0.3 V, which was negatively shifted compared with that at bare GCE or Aunano/GCE, illustrating better catalytic performance of rGO-Aunano.
After the modification of GLUD, the GLUD/rGO-Aunano/GCE led to effective amperometric detection of α-KG through monitoring the NADH consumption and displayed a linear response in the range of 66.7 and 494.5 μM, with the detection limit of 9.2 μM.
Moreover, the prepared GLUD/rGO-Aunano/GCE was further evaluated to be highly selective and used to test α-KG in human serum samples.
The recovery and the RSD values were calculated in the range of 97.9–102.4% and 3.8–4.5%, respectively, showing a great prospect for its real application.
American Psychological Association (APA)
Peng, Gang& Yu, Yadong& Chen, Xiaojun& Huang, He. 2020. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
Modern Language Association (MLA)
Peng, Gang…[et al.]. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
American Medical Association (AMA)
Peng, Gang& Yu, Yadong& Chen, Xiaojun& Huang, He. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167653