Comprehensive Screening and Identification of Phillyrin Metabolites in Rats Based on UHPLC-Q-Exactive Mass Spectrometry Combined with Multi-Channel Data Mining
Joint Authors
Ma, Beibei
Li, Jiameng
Lou, Tianyu
Liang, Yaoyue
Wang, Chenxiao
Li, Ruiji
Wang, Tingting
Liu, Jinhui
Guo, Yudong
Wang, Zhibin
Wang, Jing
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-24
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
Phillyrin, a well-known bisepoxylignan, has been shown to have many critical pharmacological activities.
In this study, a novel strategy for the extensive acquisition and use of data was established based on UHPLC-Q-Exactive mass spectrometry to analyze and identify the in vivo metabolites of phillyrin and to elucidate the in vivo metabolic pathways of phillyrin.
Among them, the generation of data sets was mainly due to multichannel data mining methods, such as high extracted ion chromatogram (HEIC), diagnostic product ion (DPI), and neutral loss filtering (NLF).
A total of 60 metabolites (including the prototype compound) were identified in positive and negative ion modes based on intuitive and useful data such as the standard’s cleavage rule, accurate molecular mass, and chromatographic retention time.
The results showed that a series of biological reactions of phillyrin in vivo mainly included methylation, hydroxylation, hydrogenation, sulfonation, glucuronidation, demethylation, and dehydrogenation and their composite reactions.
In summary, this study not only comprehensively explained the in vivo metabolism of phillyrin, but also proposed an effective strategy to quickly analyze and identify the metabolites of natural pharmaceutical ingredients in nature.
American Psychological Association (APA)
Ma, Beibei& Li, Jiameng& Lou, Tianyu& Liang, Yaoyue& Wang, Chenxiao& Li, Ruiji…[et al.]. 2020. Comprehensive Screening and Identification of Phillyrin Metabolites in Rats Based on UHPLC-Q-Exactive Mass Spectrometry Combined with Multi-Channel Data Mining. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1167698
Modern Language Association (MLA)
Ma, Beibei…[et al.]. Comprehensive Screening and Identification of Phillyrin Metabolites in Rats Based on UHPLC-Q-Exactive Mass Spectrometry Combined with Multi-Channel Data Mining. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-20.
https://search.emarefa.net/detail/BIM-1167698
American Medical Association (AMA)
Ma, Beibei& Li, Jiameng& Lou, Tianyu& Liang, Yaoyue& Wang, Chenxiao& Li, Ruiji…[et al.]. Comprehensive Screening and Identification of Phillyrin Metabolites in Rats Based on UHPLC-Q-Exactive Mass Spectrometry Combined with Multi-Channel Data Mining. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-20.
https://search.emarefa.net/detail/BIM-1167698
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167698