Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS

Joint Authors

An, Lijun
Yu, Xie-An
Li, Jin
Chang, Yan-Xu
Liu, Wei

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-11-17

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Chemistry
Science

Abstract EN

Neuraminidase plays an essential role in the spread of influenza viruses via cleaving sialic acids from the host cell receptors and virions.

Neuraminidase has been regarded as an essential target for prevention and treatment of influenza infection.

The one-step high-performance liquid chromatography-fraction collector (HPLC-FC) was selected to prepare fractions from Reduning (RDN) injection, while ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was used to identify fractions depending on their retention time and molecular ion.

As a result, 75 fractions were prepared and 28 fractions out of them exhibited NA inhibitory effects with the dose-effect relationship.

Exploring it further, six components including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C were the main components that accounted for almost 80% of inhibitory activity of RDN injection.

Accordingly, these results demonstrated that this strategy could not only rapidly identify but also accurately screen active components from traditional Chinese medicine.

American Psychological Association (APA)

An, Lijun& Yu, Xie-An& Liu, Wei& Li, Jin& Chang, Yan-Xu. 2020. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1167755

Modern Language Association (MLA)

Liu, Wei…[et al.]. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1167755

American Medical Association (AMA)

An, Lijun& Yu, Xie-An& Liu, Wei& Li, Jin& Chang, Yan-Xu. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1167755

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167755