Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS
Joint Authors
An, Lijun
Yu, Xie-An
Li, Jin
Chang, Yan-Xu
Liu, Wei
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Neuraminidase plays an essential role in the spread of influenza viruses via cleaving sialic acids from the host cell receptors and virions.
Neuraminidase has been regarded as an essential target for prevention and treatment of influenza infection.
The one-step high-performance liquid chromatography-fraction collector (HPLC-FC) was selected to prepare fractions from Reduning (RDN) injection, while ultra-high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was used to identify fractions depending on their retention time and molecular ion.
As a result, 75 fractions were prepared and 28 fractions out of them exhibited NA inhibitory effects with the dose-effect relationship.
Exploring it further, six components including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C were the main components that accounted for almost 80% of inhibitory activity of RDN injection.
Accordingly, these results demonstrated that this strategy could not only rapidly identify but also accurately screen active components from traditional Chinese medicine.
American Psychological Association (APA)
An, Lijun& Yu, Xie-An& Liu, Wei& Li, Jin& Chang, Yan-Xu. 2020. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1167755
Modern Language Association (MLA)
An, Lijun…[et al.]. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1167755
American Medical Association (AMA)
An, Lijun& Yu, Xie-An& Liu, Wei& Li, Jin& Chang, Yan-Xu. Identification and Screening of Natural Neuraminidase Inhibitors from Reduning Injection via One-Step High-Performance Liquid Chromatography-Fraction Collector and UHPLCQ-TOF-MS. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1167755
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167755