A Validated Method for the Simultaneous Determination of Methamphetamine and 3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem Mass Spectrometry
Joint Authors
Le, Dinh-Vu
Nguyen, Trong-Tuan
Nguyen, Van-Trong
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been validated for the simultaneous determination of methamphetamine (MA) and 3,4-methylenedioxy-N-methamphetamine (MDMA) in the blood sample.
Under the optimal experimental conditions, the concentration of MA can be determined in the range from 1 µg/L to 5000 µg/L with the method detection limit (MDL) of 0.31 µg/L.
The range from 0.5 to 500 µg/L is observed for the determination of MDMA with the MDL down to 0.25 µg/L.
The practical applicability of the method is performed with the recovery ranging from 85.3% to 94% for MA and from 86.9% to 95.5% for MDMA.
At the different concentrations of drugs, the relative standard deviations (RSD) for both MA and MDMA are lower than 5.7%.
The method was applied to analyse 1995 blood samples that had been collected from the Forensic Medicine Centre of Ho Chi Minh City.
The results showed 1.75% positive with MA and 0.25% positive with MDMA.
These two drugs take 10% of the total drugs positive samples.
By using deuterium-labelled methamphetamine-d5 and 3,4-methylenedioxy-N-methamphetamine-d5 as the internal standards in the determination and the use of MS/MS in multiple reaction monitoring mode signal readout, the method exhibits robustness specificity and can be applied in simultaneous determination of MA and MDMA in blood with high selectivity and sensitivity.
American Psychological Association (APA)
Le, Dinh-Vu& Nguyen, Trong-Tuan& Nguyen, Van-Trong. 2020. A Validated Method for the Simultaneous Determination of Methamphetamine and 3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem Mass Spectrometry. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167772
Modern Language Association (MLA)
Le, Dinh-Vu…[et al.]. A Validated Method for the Simultaneous Determination of Methamphetamine and 3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem Mass Spectrometry. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167772
American Medical Association (AMA)
Le, Dinh-Vu& Nguyen, Trong-Tuan& Nguyen, Van-Trong. A Validated Method for the Simultaneous Determination of Methamphetamine and 3,4-Methylenedioxy-N-methamphetamine in Blood-Based Liquid Chromatography-Tandem Mass Spectrometry. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167772