Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study
Joint Authors
Escobar, Lina M.
Bendahan, Zita
Bayona, Andrea
González, María-Clara
Castellanos, Jaime E.
Source
International Journal of Dentistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs).
Methods.
In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods.
Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively.
Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR.
Results.
Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h.
Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment.
After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes.
Conclusions.
Vitamins D and E induced osteoblastic differentiation of hDPSCs, as evidenced by the decrease in cell proliferation, morphological changes, and the formation of calcified nodules, increasing the expression of differentiation genes.
Concurrent treatment with vitamins D + E induces a synergistic effect in differentiation toward an osteoblastic lineage.
American Psychological Association (APA)
Escobar, Lina M.& Bendahan, Zita& Bayona, Andrea& Castellanos, Jaime E.& González, María-Clara. 2020. Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study. International Journal of Dentistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1169795
Modern Language Association (MLA)
Escobar, Lina M.…[et al.]. Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study. International Journal of Dentistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1169795
American Medical Association (AMA)
Escobar, Lina M.& Bendahan, Zita& Bayona, Andrea& Castellanos, Jaime E.& González, María-Clara. Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study. International Journal of Dentistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1169795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169795