“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging
Joint Authors
Meng, Xiang-ying
Yi, Zhengjun
Qiao, Jinjuan
Zhao, Ronglan
Chu, Hairong
Wang, Ying
Chen, Xiangyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In this work, a sensitive and selective turn-on fluorimetric method has been developed for the determination of biothiols based on blocking Ag+-induced fluorescence quenching of nitrogen-rich polymer carbon nanostrips (NRPCNSs).
Ag+ ion can induce the fluorescence quenching of NRPCNSs due to the formation of nonfluorescent coordination complexes via robust Ag-N interaction.
Once addition of biothiols, such as cysteine (Cys) and glutathione (GSH), Ag+ ions prefer to interact with biothiols rather than NRPCNSs, which could be attribute to the formation of Ag-S bond, thus leading to effective fluorescent recovery of NRPCNSs.
Under the optimized conditions, excellent linear relationships existed between the recovery degree of the NRPCNSs and the concentrations of Cys and GSH in the range of 0.05 μM to 10 μM and 0.2 μM to 30 μM, respectively.
And, the limits of detection (LODs) for Cys and GSH are 16.5 nM and 65.1 nM, respectively.
The detection system also shows high selectivity against other non-thiol amino acids.
Moreover, the potential in practical applications of this proposed method has been demonstrated by detecting biothiols in human serum and fluorescence imaging of biothiols in living cells.
American Psychological Association (APA)
Meng, Xiang-ying& Qiao, Jinjuan& Zhao, Ronglan& Chu, Hairong& Wang, Ying& Chen, Xiangyu…[et al.]. 2019. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
Modern Language Association (MLA)
Meng, Xiang-ying…[et al.]. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
American Medical Association (AMA)
Meng, Xiang-ying& Qiao, Jinjuan& Zhao, Ronglan& Chu, Hairong& Wang, Ying& Chen, Xiangyu…[et al.]. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1170775