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Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell
Joint Authors
Zhang, Chengshuo
Zhang, Jialin
Jiao, Ao
Li, Feng
Lv, W.
Chen, Baomin
Source
International Journal of Endocrinology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cholinergic neurons can functionally support pancreatic islets in controlling blood sugar levels.
However, in islet transplantation, the level of cholinergic reinnervation is significantly lower compared to orthotopic pancreatic islets.
This abnormal reinnervation affects the survival and function of islet grafts.
In this study, the cholinergic reinnervation of beta cells was simulated by 2D and 3D coculture of INS-1 and NG108-15 cells.
In 2D culture conditions, 20 mM glucose induced a 1.24-fold increase (p<0.0001) in insulin secretion from the coculture group, while in the 3D culture condition, a 1.78-fold increase (p<0.0001) in insulin secretion from heterotypic pseudoislet group was observed.
Glucose-stimulated insulin secretion (GSIS) from 2D INS-1 cells showed minimal changes when compared to 3D structures.
E-cadherin expressed in INS-1 and NG108-15 cells was the key adhesion molecule for the formation of heterotypic pseudoislets.
NG108-15 cells hardly affected the proliferation of INS-1 cells in vitro.
Heterotypic pseudoislet transplantation recipient mice reverted to normoglycemic levels faster and had a greater blood glucose clearance compared to INS-1 pseudoislet recipient mice.
In conclusion, cholinergic cells can promote insulin-secreting cells to function better in vitro and in vivo and E-cadherin plays an important role in the formation of heterotypic pseudoislets.
American Psychological Association (APA)
Jiao, Ao& Li, Feng& Zhang, Chengshuo& Lv, W.& Chen, Baomin& Zhang, Jialin. 2018. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
Modern Language Association (MLA)
Jiao, Ao…[et al.]. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
American Medical Association (AMA)
Jiao, Ao& Li, Feng& Zhang, Chengshuo& Lv, W.& Chen, Baomin& Zhang, Jialin. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171024