HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway
Joint Authors
Tan, Jiaorong
Xu, Jiahong
Wei, Guohua
Zhang, Lijuan
Sun, Long’e
Wang, Guangyu
Li, Fei
Jiang, Fengxiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
This study is aimed at evaluating the effects, functions, and mechanism of HNF1α on hepatic glycolipid metabolism.
In this study, free fatty acid- (FFA-) induced steatosis of hepatocyte liver cell LO2 was used as an in vitro model.
The methods of Oil Red O staining, RT-qPCR, western blot, and immunofluorescence staining were used to detect LO2-regulated HNF1α expression and its effects on FFA-induced LO2 cell steatosis, the insulin signaling and SOCS-3-STAT3 signaling pathways, the expression of lipid metabolism-related regulators, and phosphorylation.
With increased FFA induction time, the expression of HNF1α in the LO2 fatty degeneration hepatic cells gradually decreased.
Downregulation of HNF1α expression aggravated FFA-induced steatosis of LO2 hepatocytes.
HNF1α promotes activation of the insulin pathway and oxidative breakdown of fat and inhibits lipid anabolism.
Inhibitors of STAT3 can reverse the regulation of decreased HNF1α expression on the insulin signaling pathway and fat metabolism.
We also confirmed this pathway using HNF1α-/- mice combining treatment with STAT3 inhibitor NSC 74859 in vivo.
HNF1α regulates hepatic lipid metabolism by promoting the expression of SOCS-3 and negatively regulating the STAT3 signaling pathway.
American Psychological Association (APA)
Tan, Jiaorong& Xu, Jiahong& Wei, Guohua& Zhang, Lijuan& Sun, Long’e& Wang, Guangyu…[et al.]. 2019. HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1173043
Modern Language Association (MLA)
Tan, Jiaorong…[et al.]. HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway. Journal of Diabetes Research No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1173043
American Medical Association (AMA)
Tan, Jiaorong& Xu, Jiahong& Wei, Guohua& Zhang, Lijuan& Sun, Long’e& Wang, Guangyu…[et al.]. HNF1α Controls Liver Lipid Metabolism and Insulin Resistance via Negatively Regulating the SOCS-3-STAT3 Signaling Pathway. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1173043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173043