Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method

Joint Authors

Li, Mingming
Gao, Shouhong
Chen, Wansheng
Wang, Zhipeng
Wei, Hua
Zhong, Renqian
Zhang, Feng
Yang, Yang

Source

Journal of Analytical Methods in Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-01

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Chemistry

Abstract EN

A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients.

The chromatographic separation was achieved on an Agilent ZORBAX SB-C18 column (3.0 × 150 mm, 5 μm) with a binary gradient elution system (A, 0.02% heptafluorobutyric acid and 0.2% formic acid in water, v/v; B, methanol).

The run time was 10 min.

The multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition.

The linear correlation coefficients were >0.99 for all the analytes in their corresponding calibration ranges.

The sample was pretreated based on tissue homogenate and protein precipitation with a 100 mg aliquot sample.

The average recovery and matrix effect for 34 amino acids and 3 internal standards were 39.00%∼146.95% and 49.45%∼173.63%, respectively.

The intra- and interday accuracy for all the analytes ranged from −13.52% to 14.21% (RSD ≤8.57%) and from −14.52% to 12.59% (RSD ≤10.31%), respectively.

Deviations of stability under different conditions were within ±15% for all the analytes.

This method was applied to simultaneous quantification of 34 amino acids in tissue samples from 94 CRC patients.

American Psychological Association (APA)

Yang, Yang& Zhang, Feng& Gao, Shouhong& Wang, Zhipeng& Li, Mingming& Wei, Hua…[et al.]. 2020. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1174749

Modern Language Association (MLA)

Yang, Yang…[et al.]. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1174749

American Medical Association (AMA)

Yang, Yang& Zhang, Feng& Gao, Shouhong& Wang, Zhipeng& Li, Mingming& Wei, Hua…[et al.]. Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MSMS Method. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1174749

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1174749