Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats
Joint Authors
Li, Xiaoxin
He, Aiping
Zhou, Chunling
Tian, Jinmiao
Jiang, Meihua
Fang, Lina
Liu, Tianzhu
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-17
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
VS-5584 is a small-molecular compound that showed equivalent activity against mTOR and all class I PI3K isoforms and demonstrated preclinical activity in diverse cancer cell lines and xenograft tumor model, and rational combination of VS-5584 and other target therapies achieved promising outcomes in oncology.
In the present study, we established and validated a simple and sensitive UPLC-MS/MS method for the determination of VS-5584 in plasma samples.
VS-5584 was separated via an Acquity UPLC BEH C18 column, with a mobile phase composed of acetonitrile and 0.2% formic acid in water (40 : 60).
The calibration curve displayed a good linearity in the range of 1.0–1000 ng/mL, with satisfactory accuracy (−13.6% < RE% < 8.8%) and precision (CV%, less than 9.2%).
The validated method was then applied to a pharmacokinetic study in rats.
After administration of 10 mg/kg, VS-5584 was absorbed quickly and reached a peak concentration of 473.2 ± 72.0 ng/mL after 20 min.
The established method allows for the quantification of VS-5584 in rat plasma in detail and can be utilized to successfully describe the pharmacokinetic profile of VS-5584.
American Psychological Association (APA)
Zhou, Chunling& Li, Xiaoxin& He, Aiping& Liu, Tianzhu& Tian, Jinmiao& Jiang, Meihua…[et al.]. 2020. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
Modern Language Association (MLA)
Zhou, Chunling…[et al.]. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
American Medical Association (AMA)
Zhou, Chunling& Li, Xiaoxin& He, Aiping& Liu, Tianzhu& Tian, Jinmiao& Jiang, Meihua…[et al.]. Development of a UPLC-MSMS Method for the Quantification of VS-5584 and Its Application in Pharmacokinetic Studies in Rats. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174892
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174892