Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

Joint Authors

Zhao, Qiaoling
Yin, Congcong
Yue, Aiqin
Du, Weijun
Liu, Dingbin
Zhao, Jinzhong
Zhang, Yongpo
Wang, Min

Source

Journal of Analytical Methods in Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-11-05

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Chemistry

Abstract EN

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health.

GmSg-1 gene is the key enzyme gene for synthesizing class A saponins.

It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level.

The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes.

The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM).

It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

American Psychological Association (APA)

Yin, Congcong& Zhao, Qiaoling& Yue, Aiqin& Du, Weijun& Liu, Dingbin& Wang, Min…[et al.]. 2020. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174895

Modern Language Association (MLA)

Wang, Min…[et al.]. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1174895

American Medical Association (AMA)

Yin, Congcong& Zhao, Qiaoling& Yue, Aiqin& Du, Weijun& Liu, Dingbin& Wang, Min…[et al.]. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174895

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1174895