Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction
Joint Authors
Zhao, Qiaoling
Yin, Congcong
Yue, Aiqin
Du, Weijun
Liu, Dingbin
Zhao, Jinzhong
Zhang, Yongpo
Wang, Min
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health.
GmSg-1 gene is the key enzyme gene for synthesizing class A saponins.
It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level.
The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes.
The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM).
It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.
American Psychological Association (APA)
Yin, Congcong& Zhao, Qiaoling& Yue, Aiqin& Du, Weijun& Liu, Dingbin& Zhao, Jinzhong…[et al.]. 2020. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174895
Modern Language Association (MLA)
Yin, Congcong…[et al.]. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1174895
American Medical Association (AMA)
Yin, Congcong& Zhao, Qiaoling& Yue, Aiqin& Du, Weijun& Liu, Dingbin& Zhao, Jinzhong…[et al.]. Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174895