Simple and Selective HPLC-UVVis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies
Joint Authors
Cunha-Filho, Marcilio S. S.
Bentes de Azevedo, Ricardo
Reis, Thaiene Avila
Gratieri, Taís
Joanitti, Graziella A.
Jaculi, Ana Elise
Alves, Rubens da Costa
Gelfuso, Guilherme Martins
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Considering the feasibility of the aluminum phthalocyanine chloride (AlPcCl) application in the topical photodynamic therapy of cutaneous tumors and the lack of HPLC methods capable of supporting skin permeation experiments using this compound, the aim of this study was to obtain a simple and selective chromatographic method for AlPcCl determination in skin matrices.
A HPLC-UV/Vis method was developed using a normal-phase column operating at 30°C, an isocratic mobile phase of methanol : phosphoric acid (0.01 M) at 1.5 mL/min, and detection at 670 nm.
The method exhibited (i) selectivity against various contaminants found in the different skin layers, (ii) high drug extraction capacity from the hair follicle (>70%) and remaining skin (>80%), and (iii) low limits of detection and of quantification (0.03 and 0.09 μg/mL, resp.).
The method was also linear in the range from 0.1 to 5.0 µg/mL (r = 0.9994) and demonstrated robustness with regard to experimental chromatographic parameters according to a factorial design.
Lastly, the developed method was successfully tested in in vitro skin permeation studies of AlPcCl, proving its effectiveness in the development of pharmaceutical delivery systems containing this drug for topical photodynamic therapy of skin cancers.
American Psychological Association (APA)
Reis, Thaiene Avila& Jaculi, Ana Elise& Alves, Rubens da Costa& Gratieri, Taís& Bentes de Azevedo, Ricardo& Joanitti, Graziella A.…[et al.]. 2018. Simple and Selective HPLC-UVVis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176492
Modern Language Association (MLA)
Reis, Thaiene Avila…[et al.]. Simple and Selective HPLC-UVVis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176492
American Medical Association (AMA)
Reis, Thaiene Avila& Jaculi, Ana Elise& Alves, Rubens da Costa& Gratieri, Taís& Bentes de Azevedo, Ricardo& Joanitti, Graziella A.…[et al.]. Simple and Selective HPLC-UVVis Bioanalytical Method to Determine Aluminum Phthalocyanine Chloride in Skin Permeation Studies. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176492