Apolipoprotein C-II Mimetic Peptide Promotes the Plasma Clearance of Triglyceride-Rich Lipid Emulsion and the Incorporation of Fatty Acids into Peripheral Tissues of Mice

Joint Authors

Remaley, Alan T.
Wolska, Anna
Komatsu, Tomohiro
Sakurai, Toshihiro
Amar, Marcelo J.
Sakurai, Akiko
Vaisman, Boris L.
Sviridov, Denis
Demosky, Stephen
Pryor, Milton
Ikewaki, Katsunori

Source

Journal of Nutrition and Metabolism

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-02-03

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Nutrition & Dietetics

Abstract EN

Aim.

Plasma apolipoprotein C-II (apoC-II) activates lipoprotein lipase (LPL) and thus lowers plasma triglycerides (TG).

We previously reported that a human apoC-II mimetic peptide (C-II-a) decreased plasma TG in apoC-II mutant mice, as well as in apoE-knockout mice.

Because it is unknown what tissues take up free fatty acids (FFAs) released from TG after C-II-a peptide administration, we investigated in mice TG plasma clearance and tissue incorporation, using 3H-triolein as a tracer, with and without C-II-a treatment.

Methods and Results.

Intralipid® fat emulsion was labeled with 3H-triolein and then mixed with or without C-II-a.

Addition of the peptide did not alter mean particle size of the lipid emulsion particles (298 nm) but accelerated their plasma clearance.

After intravenous injection into C57BL/6N mice, the plasma half-life of the 3H-triolein for control and C-II-a treated emulsions was 18.3 ± 2.2 min and 14.8 ± 0.1 min, respectively.

In apoC-II mutant mice, the plasma half-life of 3H-triolein for injected control and C-II-a treated emulsions was 30.1 ± 0.1 min and 14.8 ± 0.1 min, respectively.

C57BL/6N and apoC-II mutant mice at 120 minutes after the injection showed increased tissue incorporation of radioactivity in white adipose tissue when C-II-a treated emulsion was used.

Higher radiolabeled uptake of lipids from C-II-a treated emulsion was also observed in the skeletal muscle of C57BL/6N mice only.

In case of apoC-II mutant mice, decreased uptake of radioactive lipids was observed in the liver and kidney after addition of C-II-a to the lipid emulsion.

Conclusions.

C-II-a peptide promotes the plasma clearance of TG-rich lipid emulsions in wild type and apoC-II mutant mice and promotes the incorporation of fatty acids from TG in the lipid emulsions into specific peripheral tissues.

American Psychological Association (APA)

Komatsu, Tomohiro& Sakurai, Toshihiro& Wolska, Anna& Amar, Marcelo J.& Sakurai, Akiko& Vaisman, Boris L.…[et al.]. 2019. Apolipoprotein C-II Mimetic Peptide Promotes the Plasma Clearance of Triglyceride-Rich Lipid Emulsion and the Incorporation of Fatty Acids into Peripheral Tissues of Mice. Journal of Nutrition and Metabolism،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1183657

Modern Language Association (MLA)

Komatsu, Tomohiro…[et al.]. Apolipoprotein C-II Mimetic Peptide Promotes the Plasma Clearance of Triglyceride-Rich Lipid Emulsion and the Incorporation of Fatty Acids into Peripheral Tissues of Mice. Journal of Nutrition and Metabolism No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1183657

American Medical Association (AMA)

Komatsu, Tomohiro& Sakurai, Toshihiro& Wolska, Anna& Amar, Marcelo J.& Sakurai, Akiko& Vaisman, Boris L.…[et al.]. Apolipoprotein C-II Mimetic Peptide Promotes the Plasma Clearance of Triglyceride-Rich Lipid Emulsion and the Incorporation of Fatty Acids into Peripheral Tissues of Mice. Journal of Nutrition and Metabolism. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1183657

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1183657