Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats
Joint Authors
Kanamoto, Takashi
Ikeda, Yasuhiro
Asaoka, Ryo
Kitaoka, Yasushi
Tachibana, Takashi
Hisatomi, Toshio
Murakami, Yusuke
Tobiume, Kei
Kiuchi, Yoshiaki
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins.
Methods.
Adult Wistar rats with ocular hypertension were used as an experimental model.
D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis.
The concentration of ATP was measured by ELISA.
Results.
D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats.
The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas.
Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats.
Conclusion.
Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.
American Psychological Association (APA)
Kanamoto, Takashi& Tachibana, Takashi& Kitaoka, Yasushi& Hisatomi, Toshio& Ikeda, Yasuhiro& Murakami, Yusuke…[et al.]. 2019. Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1185229
Modern Language Association (MLA)
Kanamoto, Takashi…[et al.]. Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats. Journal of Ophthalmology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1185229
American Medical Association (AMA)
Kanamoto, Takashi& Tachibana, Takashi& Kitaoka, Yasushi& Hisatomi, Toshio& Ikeda, Yasuhiro& Murakami, Yusuke…[et al.]. Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1185229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185229