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The Protective Effects of αB-Crystallin on Ischemia-Reperfusion Injury in the Rat Retina
Joint Authors
Yan, Huan
Peng, Yanli
Huang, Wei
Gong, Liyan
Li, Li
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
To investigate whether αB-crystallin protects against acute retinal ischemic reperfusion injury (I/R) and elucidate the potential antioxidant mechanisms.
Retinal I/R injury was made by elevating the intraocular pressure (IOP) 110 mmHg for 60 min, and αB-crystallin (1 × 10−5 g/L) or vehicle solution was administered intravitreously immediately after I/R injury.
The animal was sacrificed 24 h, 1 w, and 1 m after the I/R injury.
The retina damage was detected by hematoxylin and eosin (HE) staining and electroretinography (ERG).
The level of malondialdehyde (MDA), nitric oxide (NO), and the total superoxide dismutase (T-SOD) was determined.
An immunohistochemical study was performed to detect the activation of inducible nitric oxide synthase (iNOS) and NF- (nuclear factor-) kappaB (NF-κB) p65.
The decrease of retinal thickness and the number of retinal ganglion cells (RGCs) can be suppressed by αB-crystallin.
And the amplitudes of a- and b-wave were remarkably greater without αB-crystallin.
Similarly, αB-crystallin also significantly decreased the level of MDA and NO and enhanced the activities of T-SOD.
The positive expression of iNOS and NF-kappaB p65 was obviously reduced while treated with αB-crystallin.
αB-crystallin can inhibit the expression of NF-κB and its antioxidative effect to protect the retina from I/R injury.
American Psychological Association (APA)
Yan, Huan& Peng, Yanli& Huang, Wei& Gong, Liyan& Li, Li. 2017. The Protective Effects of αB-Crystallin on Ischemia-Reperfusion Injury in the Rat Retina. Journal of Ophthalmology،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1185300
Modern Language Association (MLA)
Yan, Huan…[et al.]. The Protective Effects of αB-Crystallin on Ischemia-Reperfusion Injury in the Rat Retina. Journal of Ophthalmology No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1185300
American Medical Association (AMA)
Yan, Huan& Peng, Yanli& Huang, Wei& Gong, Liyan& Li, Li. The Protective Effects of αB-Crystallin on Ischemia-Reperfusion Injury in the Rat Retina. Journal of Ophthalmology. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1185300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185300