Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract
Joint Authors
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Uropathogenic Escherichia coli (UPEC) adhere to cells in the human urinary tract via type 1 pili that undergo phase variation where a 314-bp fimS DNA element flips between Phase-ON and Phase-OFF orientations through two site-specific recombinases, FimB and FimE.
Three fim-lux operon transcriptional fusions were created and moved into the clinical UPEC isolate NU149 to determine their temporal regulation in UPEC growing in the urinary tract.
Within murine urinary tracts, the UPEC strains demonstrated elevated transcription of fimA and fimB early in the infection, but lower transcription by the fifth day in murine kidneys.
In contrast, fimE transcription was much lower than either fimA or fimB early, increased markedly at 24 h after inoculation, and then dropped five days after inoculation.
Positioning of fimS was primarily in the Phase-ON position over the time span in UPEC infected bladders, whereas in UPEC infected murine kidneys the Phase-OFF orientation was favored by the fifth day after inoculation.
Hemagglutination titers with guinea pig erythrocytes remained constant in UPEC growing in infected murine bladders but fell substantially in UPEC infected kidneys over time.
Our results show temporal in vivo regulation of fim gene expression in different environmental niches when UPEC infects the murine urinary tract.
American Psychological Association (APA)
William, Schwan& Ding, Hua. 2017. Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract. Journal of Pathogens،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1186143
Modern Language Association (MLA)
William, Schwan& Ding, Hua. Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract. Journal of Pathogens No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1186143
American Medical Association (AMA)
William, Schwan& Ding, Hua. Temporal Regulation of fim Genes in Uropathogenic Escherichia coli during Infection of the Murine Urinary Tract. Journal of Pathogens. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1186143
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186143