Selenium Nanoparticles Induce Potent Protective Immune Responses against Vibrio cholerae WC Vaccine in a Mouse Model

Joint Authors

Raahati, Zahra
Bakhshi, Bita
Najar-peerayeh, Shahin

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-30

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Biology

Abstract EN

The aim of this study was to evaluate the efficacy of selenium nanoparticle (an immune booster) and naloxone (an opioid receptor antagonist) as a new adjuvant in increasing immune responses against killed whole-cell Vibrio cholerae in a mouse cholera model.

The Se NPs were synthesized and characterized by UV-visible, DLS, and zeta potential analysis.

The SEM image confirmed the uniformity of spherical morphology of nanoparticle shape with 34 nm in size.

The concentration of the Se NPs was calculated as 0.654 μg/ml in the ICP method.

The cytotoxic activity of Se NPs on Caco-2 cells was assessed by the MTT assay and revealed 82.05% viability of cells after 24 h exposure with 100 μg/ml of Se NPs.

Female BALB/C mice were orally immunized three times on days 0, 14, and 28, and challenge experiments were performed on immunized neonates with toxigenic V.

cholerae.

Administration of Se NP diet led to significant increase in V.

cholerae-specific IgG and IgA responses in serum and saliva and caused protective immunity and 83.3% survival in challenge experiment against 1 LD50 V.

cholerae in a group receiving diet of Se NPs compared with other groups including Dukoral vaccine.

The IL-4 and IL-5 were significantly increased in response to WC+daily diet of Se NPs with or without naloxone.

Naloxone proved no effect on IL-4 and IL-5 increase and is proposed as null in the cytokine and antibody production process.

These results reveal that daily diet of Se NPs could efficiently induce immune cell effectors in both humoral and mucosal levels.

American Psychological Association (APA)

Raahati, Zahra& Bakhshi, Bita& Najar-peerayeh, Shahin. 2020. Selenium Nanoparticles Induce Potent Protective Immune Responses against Vibrio cholerae WC Vaccine in a Mouse Model. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187630

Modern Language Association (MLA)

Raahati, Zahra…[et al.]. Selenium Nanoparticles Induce Potent Protective Immune Responses against Vibrio cholerae WC Vaccine in a Mouse Model. Journal of Immunology Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1187630

American Medical Association (AMA)

Raahati, Zahra& Bakhshi, Bita& Najar-peerayeh, Shahin. Selenium Nanoparticles Induce Potent Protective Immune Responses against Vibrio cholerae WC Vaccine in a Mouse Model. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187630

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1187630