The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity
Joint Authors
He, Zheng-Wen
Zhu, Li
Wang, Huaizhou
Wu, Yu
Shen, Qian
Qin, Yang-Hua
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Rheumatoid arthritis (RA) is a complex and not fully understood autoimmune disease associated with multijoint damage.
The main effector cells, the synovial fibroblasts, are apoptosis resistant and hyperplastic which indicate that autophagy level is high in synovial tissue.
Real-time PCR, immunocytochemistry, and western blotting were used in this paper to study the autophagy status of the synovial tissues obtained from RA and OA patients at the time of joint replacement surgery.
We further evaluated the correlation between autophagy levels with RA activity-associated serum markers with SPSS.
The results showed that the expression levels (both in mRNA and in protein level) of autophagy-related proteins (belcin1, Atg5, and LC3) in the synovial tissue of patients with active rheumatoid arthritis (n=20) were significantly higher than those in OA patients (n=16).
We further showed that the LC3-II/β-actin relative gray value was strongly correlated with the serum levels of several RA activity-related markers: CRP, ESR, CCP, and RF.
Our results indicate that evaluating the autophagy level of synovial biopsies might be a useful way to diagnose RA and to estimate the disease activity.
Reducing the expression level of autophagy-related genes might become a new therapeutic target for active rheumatoid arthritis.
American Psychological Association (APA)
Zhu, Li& Wang, Huaizhou& Wu, Yu& He, Zheng-Wen& Qin, Yang-Hua& Shen, Qian. 2017. The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188688
Modern Language Association (MLA)
Zhu, Li…[et al.]. The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188688
American Medical Association (AMA)
Zhu, Li& Wang, Huaizhou& Wu, Yu& He, Zheng-Wen& Qin, Yang-Hua& Shen, Qian. The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188688
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188688