IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro
Joint Authors
Li, Chao
Lu, Yiping
Weng, Dong
Liu, Fangwei
Chen, Ying
Chen, Jie
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Long-term exposure to crystalline silica leads to silicosis, which is characterized by persistent lung inflammation and lung fibrosis.
Multiple immune cells have been demonstrated to participate in crystalline silica-induced immune responses.
Our previous study indicated that B10 could control lung inflammation through modulating the Th balance in experimental silicosis in mice.
However, the regulatory mechanism of B10 on CD4+ T cells is still unclear.
MACS-sorted CD19+ B cells from the three different groups were cultured with CD4+ T cells either with or without transwell insert plates to evaluate the effects of B10 on CD4+ T cells, including Teff and Treg.
B10 was eliminated by anti-CD22 application in vivo.
Flow cytometry was used to test the frequencies of CD4+ T cells, and the expressions of the related cytokines were detected by real-time PCR and CBA.
Insufficient B10 elevated the levels of proinflammatory cytokines and promoted Th responses in a way independent upon cell-cell contact in the Teff and B cell coculture system.
B10 could both increase Treg activity and enhance conversion of Teff into Treg.
Our findings demonstrated that B10 could affect Th responses by the release of IL-10, enhancing Treg functions and converting Teff into Treg.
American Psychological Association (APA)
Lu, Yiping& Liu, Fangwei& Li, Chao& Chen, Ying& Weng, Dong& Chen, Jie. 2017. IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188770
Modern Language Association (MLA)
Lu, Yiping…[et al.]. IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188770
American Medical Association (AMA)
Lu, Yiping& Liu, Fangwei& Li, Chao& Chen, Ying& Weng, Dong& Chen, Jie. IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188770