Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion

Joint Authors

Montanari, Elisa
Pimenta, Joel
Szabó, Luca
Noverraz, François
Passemard, Solène
Meier, Raphael P. H.
Meyer, Jeremy
Sidibe, Jonathan
Thomas, Aurelien
Schuurman, Henk-Jan
Gerber-Lemaire, Sandrine
Gonelle-Gispert, Carmen
Buhler, Leo H.

Source

Journal of Immunology Research

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-01-11

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Biology

Abstract EN

Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function.

Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation.

We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are beneficial on hepatocyte function.

Livers from 10 kg pigs (n=9) were harvested, and hepatocytes were isolated from liver suspensions for microencapsulation using alginate and poly(ethylene-glycol)- (PEG-) grafted alginate hydrogels, either alone or in combination with MSC.

Viability, albumin secretion, and diazepam catabolism of hepatocytes were measured for one week.

9.2 ± 3.6 × 109 hepatocytes with 95.2 ± 3.1% viability were obtained after isolation.

At day 3, free hepatocytes displayed 99% viability, whereas microencapsulation in alginate and PEG-grafted alginate decreased viability to 62% and 48%, respectively.

Albumin secretion and diazepam catabolism occurred in free and microencapsulated hepatocytes.

Coencapsulation of hepatocytes with MSC significantly improved viability and albumin secretion at days 4 and 8 (p<0.05).

Coculture with MSC significantly increased and prolonged albumin secretion.

In conclusion, we established a protocol for isolation and microencapsulation of high numbers of viable pig hepatocytes and demonstrated that the presence of MSC is beneficial for the viability and function of porcine hepatocytes.

American Psychological Association (APA)

Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. 2018. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528

Modern Language Association (MLA)

Montanari, Elisa…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1191528

American Medical Association (AMA)

Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1191528