Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion
Joint Authors
Montanari, Elisa
Pimenta, Joel
Szabó, Luca
Noverraz, François
Passemard, Solène
Meier, Raphael P. H.
Meyer, Jeremy
Sidibe, Jonathan
Thomas, Aurelien
Schuurman, Henk-Jan
Gerber-Lemaire, Sandrine
Gonelle-Gispert, Carmen
Buhler, Leo H.
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function.
Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation.
We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are beneficial on hepatocyte function.
Livers from 10 kg pigs (n=9) were harvested, and hepatocytes were isolated from liver suspensions for microencapsulation using alginate and poly(ethylene-glycol)- (PEG-) grafted alginate hydrogels, either alone or in combination with MSC.
Viability, albumin secretion, and diazepam catabolism of hepatocytes were measured for one week.
9.2 ± 3.6 × 109 hepatocytes with 95.2 ± 3.1% viability were obtained after isolation.
At day 3, free hepatocytes displayed 99% viability, whereas microencapsulation in alginate and PEG-grafted alginate decreased viability to 62% and 48%, respectively.
Albumin secretion and diazepam catabolism occurred in free and microencapsulated hepatocytes.
Coencapsulation of hepatocytes with MSC significantly improved viability and albumin secretion at days 4 and 8 (p<0.05).
Coculture with MSC significantly increased and prolonged albumin secretion.
In conclusion, we established a protocol for isolation and microencapsulation of high numbers of viable pig hepatocytes and demonstrated that the presence of MSC is beneficial for the viability and function of porcine hepatocytes.
American Psychological Association (APA)
Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. 2018. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
Modern Language Association (MLA)
Montanari, Elisa…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
American Medical Association (AMA)
Montanari, Elisa& Pimenta, Joel& Szabó, Luca& Noverraz, François& Passemard, Solène& Meier, Raphael P. H.…[et al.]. Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1191528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191528