IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M
Joint Authors
Dubey, Anisha
Botelho, Fernando
Ayaub, Ehab A.
Park, Rex
Yip, Ashley
Humbles, Allison
Kolbeck, Roland
Richards, Carl D.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-28
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The interleukin-1 family member IL-33 participates in both innate and adaptive T helper-2 immune cell responses in models of lung disease.
The IL-6-type cytokine Oncostatin M (OSM) elevates lung inflammation, Th2-skewed cytokines, alternatively activated (M2) macrophages, and eosinophils in C57Bl/6 mice in vivo.
Since OSM induces IL-33 expression, we here test the IL-33 function in OSM-mediated lung inflammation using IL-33-/- mice.
Adenoviral OSM (AdOSM) markedly induced IL-33 mRNA and protein levels in wild-type animals while IL-33 was undetectable in IL-33-/- animals.
AdOSM treatment showed recruitment of neutrophils, eosinophils, and elevated inflammatory chemokines (KC, eotaxin-1, MIP1a, and MIP1b), Th2 cytokines (IL-4/IL-5), and arginase-1 (M2 macrophage marker) whereas these responses were markedly diminished in IL-33-/- mice.
AdOSM-induced IL-33 was unaffected by IL-6-/- deficiency.
AdOSM also induced IL-33R+ ILC2 cells in the lung, while IL-6 (AdIL-6) overexpression did not.
Flow-sorted ILC2 responded in vitro to IL-33 (but not OSM or IL-6 stimulation).
Matrix remodelling genes col3A1, MMP-13, and TIMP-1 were also decreased in IL-33-/- mice.
In vitro, IL-33 upregulated expression of OSM in the RAW264.7 macrophage cell line and in bone marrow-derived macrophages.
Taken together, IL-33 is a critical mediator of OSM-driven, Th2-skewed, and M2-like responses in mouse lung inflammation and contributes in part through activation of ILC2 cells.
American Psychological Association (APA)
Botelho, Fernando& Dubey, Anisha& Ayaub, Ehab A.& Park, Rex& Yip, Ashley& Humbles, Allison…[et al.]. 2020. IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1191716
Modern Language Association (MLA)
Botelho, Fernando…[et al.]. IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M. Mediators of Inflammation No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1191716
American Medical Association (AMA)
Botelho, Fernando& Dubey, Anisha& Ayaub, Ehab A.& Park, Rex& Yip, Ashley& Humbles, Allison…[et al.]. IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1191716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191716