![](/images/graphics-bg.png)
Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways
Joint Authors
Hu, Sindy
Huang, Wen-Chung
Liou, Chian-Jiun
Shen, Szu-Chuan
Hsiao, Chien-Yu
Wu, Shu-Ju
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-16
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Cytokine-induced endothelial dysfunction leads to inflammation and vascular adhesion molecule production in retinal pigment epithelium (RPE) cells.
Inflammation is a critical mediator in retinal degeneration (RD) diseases, including age-related macular degeneration (AMD), and RD progression may be prevented through anti-inflammatory activity in RPE cells.
The flavonoid polyphenol luteolin (LU) has anti-inflammatory and antidiabetes activities, but its effects regarding retinal protection remain unknown.
Here, we examined the ability of luteolin to alleviate markers of inflammation related to RD in cytokine-primed APPE-19 cells.
We found that luteolin decreased the levels of interleukin- (IL-) 6, IL-8, soluble intercellular adhesion molecule-1 (sICAM-1), and monocyte chemoattractant protein-1 (MCP-1) and attenuated adherence of the human monocytic leukemia cell line THP-1 to IL-1β-stimulated ARPE-19 cells.
Luteolin also increased anti-inflammatory protein heme oxygenase-1 (HO-1) levels.
Interestingly, luteolin induced protein kinase B (AKT) phosphorylation, thus inhibiting nuclear factor- (NF-) κB transfer from cytoplasm into the nucleus and suppressing mitogen-activated protein kinase (MAPK) inflammatory pathways.
Furthermore, cotreatment with MAPK inhibitors and luteolin decreased inflammatory cytokine and chemokine levels, and further suppressed THP-1 adhesion.
Overall, these results provide evidence that luteolin protects ARPE-19 cells from IL-1β-stimulated increases of IL-6, IL-8, sICAM-1, and MCP-1 production by blocking the activation of MAPK and NF-κB signaling pathways, thus ameliorating the inflammatory response.
American Psychological Association (APA)
Huang, Wen-Chung& Liou, Chian-Jiun& Shen, Szu-Chuan& Hu, Sindy& Hsiao, Chien-Yu& Wu, Shu-Ju. 2020. Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1192224
Modern Language Association (MLA)
Huang, Wen-Chung…[et al.]. Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways. Mediators of Inflammation No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1192224
American Medical Association (AMA)
Huang, Wen-Chung& Liou, Chian-Jiun& Shen, Szu-Chuan& Hu, Sindy& Hsiao, Chien-Yu& Wu, Shu-Ju. Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1192224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192224