Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice
Joint Authors
Shevchenko, Marina A.
Bogorodskiy, Andrey O.
Troyanova, Natalia I.
Servuli, Ekaterina A.
Bolkhovitina, Elena L.
Büldt, Georg
Fahlke, Christoph
Gordeliy, Valentin I.
Gensch, Thomas
Borshchevskiy, Valentin I.
Sapozhnikov, Alexander M.
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Susceptibility to fungal infection is commonly associated with impaired neutrophil responses.
To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium inhalation in mouse models of drug-induced immunosuppression and antibody-mediated neutrophil depletion (neutropenia) by performing three-dimensional confocal laser-scanning microscopy of whole-mount primary bronchus specimens.
Actin staining enabled visualization of the epithelial and smooth muscle layers that mark the airway wall.
Gr-1+ or Ly6G+ neutrophils located between the epithelium and smooth muscles were considered airway wall neutrophils.
The number of airway wall neutrophils for immunocompetent, immunosuppressed, and neutropenic mice before and 6 h after A.
fumigatus infection were analyzed and compared.
Our results show that the number of conducting airway wall neutrophils in immunocompetent mice significantly increased upon inflammation, while a dramatic reduction in this number was observed following immunosuppression and neutropenia.
Interestingly, a slight increase in the infiltration of neutrophils into the airway wall was detected as a result of infection, even in immunosuppressed and neutropenic mice.
Taken together, these data indicate that neutrophils are present in intact conducting airway walls and the number elevates upon A.
fumigatus infection.
Conducting airway wall neutrophils are affected by both neutropenia and immunosuppression.
American Psychological Association (APA)
Shevchenko, Marina A.& Bogorodskiy, Andrey O.& Troyanova, Natalia I.& Servuli, Ekaterina A.& Bolkhovitina, Elena L.& Büldt, Georg…[et al.]. 2018. Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1192594
Modern Language Association (MLA)
Shevchenko, Marina A.…[et al.]. Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1192594
American Medical Association (AMA)
Shevchenko, Marina A.& Bogorodskiy, Andrey O.& Troyanova, Natalia I.& Servuli, Ekaterina A.& Bolkhovitina, Elena L.& Büldt, Georg…[et al.]. Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1192594
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192594