NF-κB-94insdel ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia
Joint Authors
Zhao, Xueyun
Hua, Mingqiang
Yu, Jie
Zhong, Chaoqin
Wang, Ruiqing
Zhang, Chen
Hou, Ming
Wang, Rui
Li, Guosheng
He, Na
Ma, Daoxin
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
The NLRP3 inflammasome plays important roles in the pathogenesis of autoimmune diseases.
However, the role of the NLRP3 inflammasome in the pathophysiology of immune thrombocytopenia (ITP) remains unclear.
Methods.
RT-PCR was used to examine the polymorphism and expression of genes involved in the NLRP3 inflammasome in ITP patients.
T helper cells and apoptosis of PBMC from ITP patients were analyzed by flow cytometry.
The antiplatelet autoantibodies in plasma were determined by modified monoclonal antibody-specific immobilization of platelet antigens (MAIPA).
Results.
We found that the NF-κB-94ins/del ATTG genotype contributed to the susceptibility of ITP.
Furthermore, the platelet counts of ITP patients with the WW genotype or WD genotype were lower than those with the DD genotype of NF-κB-94ins/del ATTG.
Compared with controls, NF-κB gene expression was significantly decreased and WW or WD genotype ITP patients displayed higher mRNA expression than DD individuals.
Similarly, the mRNA expression of NLRP3 was also increased in the WW genotype.
There was a significant gene dose effect of the percentage of Th17 cells for the WW, WD, and DD genotypes (WW < WD < DD) in the unstimulated group and no significant difference was found after being stimulated.
The activation of the NLRP3 inflammasome could upregulate Th17 in ITP patients.
Conclusion.
The NF-κB-94ins/del ATTG genotype might serve as a novel biomarker and potential target for ITP.
American Psychological Association (APA)
Yu, Jie& Hua, Mingqiang& Zhao, Xueyun& Wang, Rui& Zhong, Chaoqin& Zhang, Chen…[et al.]. 2018. NF-κB-94insdel ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193077
Modern Language Association (MLA)
Yu, Jie…[et al.]. NF-κB-94insdel ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1193077
American Medical Association (AMA)
Yu, Jie& Hua, Mingqiang& Zhao, Xueyun& Wang, Rui& Zhong, Chaoqin& Zhang, Chen…[et al.]. NF-κB-94insdel ATTG Genotype Contributes to the Susceptibility and Imbalanced Th17 Cells in Patients with Immune Thrombocytopenia. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193077
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193077